CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



43 



Bad. colt shows good growth. B. 

 enteritidis Gartner and dysentery 

 bacillus show good growth. 



<g) NH4CI 1.63 g. 



Bad. coli and B. enteritidis show 

 slight growth. Also B. 'paratyphi. 



<h) (NH4)2C03 1.1 g. 



Bad. coli and B. enteritidis show 

 slight growth. Also B. paratyphi. 



(i) Ammonium lactate 2.94 g. 



B. typhi, B. paratyphi, B. enteri- 

 tidis, Gartner, B. typhi murum and 

 dysentery show slight growth. Bad. 

 coli grows well. 



(j) Ammonium tartrate 2.62 g. 



B. paratyphi. Bad. coli, B. enteri- 

 tidis Gartner show good growth. 

 B. dys. Flexner show growth. 



(k) Asparagin 1-9 g. 



Nearly all strains show growth. 



(1) Nucleic acid (from yeast 



Merck 2.6 g. 



Nearly all except B. paratyphi A 

 show growth. 

 Reference: Kisch (1918-19 p. 32). 



130. Beijerinck's Phosphate Glucose Salt 



Solution (Harvey No. I) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.2 g. 



4. NaCl 0.02 g. 



5. 1-1000 MnS04 1.0 cc. 



6. 1-1000 FeS04 1.0 cc. 



7. Glucose 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Study of nitrogen fixation. 

 Variants : 



(a) Giltner used 0.5 g. MgS04, 0.01 g. 

 Fe2(S04)3, 0.01 g. MnS04 and 0.01 

 g. NaCl. 



(b) Heinemann used 0.5 g. MgS04, 0.01 

 g. FeS04, 0.01 g. MnS04 and 0.01 g. 

 NaCl. 



Reference: Harvey (1921-22 p. 105), Gilt- 

 ner (1921 p. 370), Heinemann (1922 p. 39). 



131. Laborde's Basal Invert Sugar Tartrate 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Invert sugar 50.0 g. 



3. Tartaric acid 2.5 g. 



4. Potassium tartrate 



(neutral) 0.75 g. 



5. Mg3(P04)2 1.0 g. 



6. H2SO4 0.1 g. 



7. Iron and zinc sulphate trace 



8. KoSiOs trace 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 



and 8 in 1. 

 Sterilization: Not specified. 

 Use: To study the nitrogen requirements 



of a mold, Eurotiopsis Gayoni. 

 Added nutrients: The author added 0.2 g. 



of nitrogen in the form of one of the 



following: 



NaNOa casein 



KNO3 asparagin 



Ammonium tar- urea 

 trate gelatin 



NH4CI fibrin 



(NH4)2S04 blood albumin 



Ammonium phos- egg albumin 

 phate yeast extract 



NH4NO2 peptone 



gluten 

 Reference: Laborde (1897 p. 3). 



132. Calmette, Massol and Breton's Basal 

 Glucose Solution 



Constituents: 



1. Water 1000.0 cc. 



2. FeS04 0.04 g. 



3. MgS04 0.05 g. 



4. K2HPO4 1.05 g. 



5. NaCl 8.5 g. 



6. Glucose 10.0 g. 



7. Na2C03 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the added nutrients to (1) . 

 Sterilization: Not specified. 



Use: To determine constituents essential 

 for growth of tubercle bacilli. The 

 authors reported that generally inorganic 

 nitrogen did not support the growth of 

 the tubercle bacillus. 



Added nutrients: The authors prepared 

 the following solutions. 



(a) Basal solution + 10.0 g. Na2C03 + 

 5.0 g. of one of the nitrogen sources. 



(b) Basal solution + 10.0 g. NaoCOs + 

 40.0 g. of glycerol + 5.0 g. of one of 

 the nitrogen sources. 



(c) Basal solution + 40.0 g. glycerol + 

 5.0 g. of one of the nitrogen sources. 



