44 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



The following nitrogen sources were 

 given : 

 NaNOs 

 NaNOa 

 (NH 4)280 4 



(NH4)2HP04 



Ammonium acetate 

 Ammonium tartrate 

 Ammonium citrate 

 Ammonium malate 

 Ammonium succinate 

 Succinamide 

 Succinimide 

 Asparagin 

 Leucin 

 Tyrosine 

 Reference: Calmette, Massol and Breton 

 (1909 p. 581). 



133. Lohnis' Basal Glucose Salt Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. MgS04 0.02 g. 



4. NaCl 0.02 g. 



5. FeoCle trace 



6. Glucose (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Dissolve 1.0% glucose and 0.1% of 

 one of the added nutrients in (1). 



Sterilization: Not specified. 



Use: To study nitrogen assimilation. 



Added nutrients: The author added 0.1% 

 of one of the following: 

 Urea (NH4)2S04 



sodium hippurate ammonium acetate 

 asparagin ammonium butyrate 



ammonium lactate NaNOs 

 uric acid 



Reference: Lohnis (1913 p. 99). 



134. Will's Basal Sucrose Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 25.0 g. 



3. MgS04 8.5 g. 



4. Sucrose 5.0 g. 



5. Citric acid 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add- 0.5% citric acid to (1). 



(3) Add one of the combinations given 

 under added nutrients. 



Sterilization: Method not given. 



Use: Cultivation of yeast. 



Added nutrients: The author added one of 



the following combinations to the basic 



solution: 



(a) asparagin 20.0 g. 



dextrin 30.0 g. 



(b) peptone 20.0 g. 



dextrin 30.0 g. 



Variants: Peklo used a solution contain- 

 ing 5.0 g. KH2PO4, 2.0 g. MgS04 and 20.0 

 g. of sucrose per liter to grow plant 

 actinomyces. 



Reference: Will (1896 p. 754), Peklo (1910 

 p. 551). 



135. Henneberg's Basal Sucrose Salt 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 (0.5%) 5.0 g. 



3. MgS04 (0.2%) 2.0 g. 



4. Sucrose 50.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Not specified. 



Use: Cultivation of yeast. 

 Added nutrients: The author added 0.3% 

 of one of the following as nitrogen sources : 

 ammonium tartrate 

 (NH4)2S04; NH4CI 

 ammonium phosphate, NH4NO3 

 Reference: Henneberg (1907 ^40-50) (1909 

 p. 104). 



136. Czapek's Sucrose Salt Solution 

 (Waksman) 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 10 g. 



3. KCl 0.5 g. 



4. MgSOi 0.5 g. 



5. FeS04 0.01 g. 



6. Sucrose 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Place in 100.0 cc. portions in flasks. 

 Sterilization : Autoclave at 15 pounds pres- 

 sure for 15 minutes. 



Use: Cultivation of bacteria found in soil. 

 Reference: Waksman (1918 p. 479). 



