CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



45 



137. von Bronsart's Basal Sucrose Salt 



Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Sucrose (4.0%) 40.0 g. 



3. MgS04 (0.25%) 2.5 g. 



4. KH2PO4 (0.25%) 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the nitrogen sources given 

 in the added nutrients. 



Sterilization: Not specified. 



Use: To study the availability of various 

 nitrogen sources for Xylaria, Xylaria 

 arbuscula, Xylaria polymorpha, Xylaria 

 hypoxylon. The author found that as- 

 paragin was a better nitrogen source 

 than peptone. KNO3 was found to be 

 the best inorganic source. 



Variants: The author used 0.5% asparagin 

 with 1.0, 4.0, 8.0, 16.0, 32.0 or 50.0% 

 sucrose. He found that sugar concen- 

 trations above 16.0% inhibited develop- 

 ment of the Xylaria listed above. There 

 was slight growth using 32.0% sucrose, 

 but no growth using 50.0% sucrose. 



Added nutrients: The author employed 

 the following nitrogen sources: 



asparagin 1 .0% 



peptone 0.5% 



NH4NO3 1.0% 



(NH4)2S04 0.5% 



Reference: von Bronsart (1919 p. 61). 



138. Bokorny's Basal Sucrose Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. MgS04 1.0 g. 



4. KH2PO4 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the materials listed under 

 added nutrients. 



Sterilization: Not specified. 



Use : To study yeast growth. 



Added nutrients and variants: One of the 

 following nitrogen sources or combina- 

 tions was added: 



(a) asparagin 25.0 g. 



(b) (NH4)2S04 5.0 g. 



(c) asparagin 5.0 g. with 1.0 g. KH2PO4, 

 a trace of CaCU and varying amounts 



of MgS04, 0.3 g. to 20.0 g. for the 

 basic solution. The author reported 

 that 2.0% MgS04 did not inhibit the 

 growth of yeast, but 0.03% sufficed 

 for normal growth. 



(d) peptone 25.0 g. 



(e) aspartic acid — amount not given, 



(f) leucine — amount not given. 



(g) tyrosine — amount not given, 

 (h) glycocoll — amount not given. 



(i) Bokorny used 4.0 g. KH2PO4 in the 

 basic solution and added one of the 

 following: 



asparagin 25.0 g. 



aspartic acid 25.0 g. 



peptone 25.0 g. 



(j) Bokorny used 4.0% sucrose in the 

 basic solution, and added 0.25% 

 aspartic acid, 

 (k) Bokorny used 1.0 g. KH2PO4, 0.3 g. 

 MgS04 and added a trace of CaCU 

 to the basic solution. To this solu- 

 tion he added: 



(a) KNO3, 0.1, 0.2 or 0.5 g. 



(b) KNO3, 0.2 and asparagin 2.0 g. 



(c) KNO3, 0.1 g. and asparagin 0.3 g. 

 Reference: Bokorny (1911 p. 183; 1912 pp. 



121; 148; 1917 p. 364; 1917 p. 47; 1920 p. 24). 



139. Smith's Sucrose Salt Solution 

 Constituents : 



1. Triple distilled water 1000.0 cc. 



2. Sucrose 5.0 cc. 



3. KH2PO4 2.0 cc. 



4. MgS04-7H20 0.1 cc. 



5. NaCl 0.5 cc. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) All apparatus should be scrupulously 

 clean and only chemicals of known 

 purity be used. 



Sterilization: Not specified. 

 Use: Cultivation of bacteria. 

 Reference: Smith (1905 p. 198), Tanner 

 a919p. 65). 



140. Miinter's Basal Galactose Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04 0.5 g. 



3. NaCl 0.5 g. 



4. K2HPO4 1.5 g. 



5. CaCl2 0.1 g. 



6. FeCl3 drops 



