46 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



7. Glucose 10.0 g. 



8. Galactose 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Distribute in 50.0 cc. lots. 



(3) Add 0.25 g. of one of the added nu- 

 trients to each 50.0 cc. lot. 



Sterilization: Not specified. 



Use: Cultivation of Actinomycetes. Actin- 

 omyces odorifer, Act. chromogenes and 

 others. Alanin and tyrosin were found 

 to be the best nitrogen sources for the 

 actinomycetes studied. The remainder 

 of the compounds, however, were good 

 sources for some of the actinomycetes. 



Added nutrients: The author added 0.25 g. 

 of one of the following to each 50.0 cc. 

 of solution: 

 urea 



sulphocarbamide 

 alanin 

 tyrosin 

 dicyandiamide 



Reference: Munter (1913 p. 377). 



141. Buchanan's Basal Maltose Salt 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. KH2PO4 2.0 g. 



3. IMgS04 0.1 g. 



4. Maltose (1.0%) 10.0 g. 



Preparation : 



(1) Add 2, 3, and 4 to 1. 



(2) Add 1.0% of one of the added nu- 

 trients. 



Sterilization: Sterilize for 20 minutes on 

 each of 3 successive days in streaming 

 steam. Then incubate at room tempera- 

 ture for one week to test sterility. 



Use : To study gum formation by Bacillus 

 radicicola. Growth and gum production 

 generally good. Poorest results were ob- 

 tained when ammonium citrate was 

 present in the medium. 



Added nutrients: The author added 1.0% 

 of one of the following: 

 Ammonium succinate 

 Ammonium citrate 

 Ammonium asparaginate 

 Asparagin 

 Peptone (Witte's) 



Reference: Buchanan (1909 p. 393). 



142. Omeliansky's Basal Cellulose Salt 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NaCl trace 



3. MgS04 0.5 g. 



4. Potassium phosphate 1-0 g. 



5. Filter paper, straw, lilac pith, or cab- 

 bage 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the added nutrients. 



(3) Add one of the cellulose sources. 

 Sterilization: Not specified. 



Use: To study fermentation of cellulose 

 by amylobacter and organisms found in 

 the mud of rivers. 



Added nutrients and variants: 



(a) The author added 1.0 g. of one of the 

 following to the basic solution: 



(NH4)2S04 



ammonium phosphate 



peptone 



asparagin 



(b) Kroulik specified the use of 1.0 g. 

 K2HPO4 in the basic solution and 

 added 1.0 to 2.0% cellulose and added 

 1.0 g. (NH4)2S04, 0.5% CaCOa, and 

 1.0% MgCOs. He used the medium 

 for the cultivation of cellulose split- 

 ting thermophiles. 



(c) Brussoff specified the use of K2HPO4 

 in the basic solution and added 1.0 g. 

 (XH4)2S04, 2.0 g. CaCOs and filter 

 paper. The solution was distributed 

 in 20.0 cc. lots and a strip of filter 

 paper (about 0.1 g.) was added to 

 each lot. The medium was used to 

 study cellulose decomposition by iron 

 bacteria {Ferribacterium duplex). 

 He reported that gas bubbles were 

 formed after 2 days and a yellow 

 membrane formed after 4 days when 

 inoculated with sludge. 



(d) Khouvine specified the use of K2HPO4 

 in the basic solution and added 1.0 g. 

 (NH4)2S04 0.0 or 2.0 g. of glucose 

 and cellulose. The medium was 

 tubed in 5.0 cc. lots and 1 square 

 centimeter of Berzilius filter paper, 

 or a little cellulose precipitated 

 after having been dissolved in 

 Schweitzer's reagent to each tube. 



