CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



47 



The medium was used for isolation 

 and enrichment of B. cellulose dis- 

 solvens. 



(e) Giltner specified the use of K2HPO4 

 in the basic solution and added 1.0 g. 

 (XH4):S04 and added 2.0 g. of filter 

 paper, cotton straw or starch. The 

 medium was used to cultivate anaer- 

 obic cellulose fermenters. 

 Reference: Omeliansky (1902 p. 226), 



Kroulik (1912-13 p. 340), Brussoff (1918 



p. 193), Giltner (1921 p. 373), Khouvine 



(1923 p. 713). 



143. Percival's Glycerol Phosphate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Magnesium phosphate 0.1 g. 



3. KH2PO4 2.0 g. 



4. Glycerol 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 4 to (1). 



(3) Heat to boiling. 



(4) Filter. 



(5) Adjustment of reaction not specified. 



(6) Tube in 10 to 15.0 cc. quantities. 



(7) After sterilization inoculate with 

 contents of a nodule of a pea, runner 

 bean, red clover and broad bean by 

 means of a sterile knife. 



Sterilization : Steam on each of 3 successive 



days. 

 Use: Growth of bacteria from the roots of 



leguminous plants, (Bacteroids). 

 Reference: Percival (1920 p. 204). 



144. Buchanan's Glycerol Salt Solution II 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 (0.2%) 2.0 g. 



3. MgSO4(0.01%) 0.1 g. 



4. Glycerol (1.0, 3.0 or 5.0%) 10.0, 30.0 or 

 50.0 g. 



Preparation : 



(1) Heat 2, 3 and 4 in 1 to boiling. 



(2) Cool on ice. 



(3) Filter to remove insoluble precipi- 

 tates. 



(4) Tube. 



Sterilization: Sterilize intermittently in 

 flowing steam on each of 3 successive days. 



Use: Cultivation of Bacillus radicicola 

 bacteroids. 



Variants: The author added (0.5%,) 5.0 g. 



ammonium phosphate with (1.0%) 10.0 g. 



glycerol. 

 Reference: Buchanan (1909 p. 62). 



145. Gerlach and Vogel's Basal Glycerol 

 Salt Solution 



Constituents: 



1. Distilled water 



2. Glycerol 



3. Potassium phosphate... 



4. AIgS04 



5. NaCl 



6. Na2C04 



7. FeS04 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add one of the nitrogen sources listed 

 in added nutrients. 



(3) Adjustment of reaction not given. 

 Sterilization: Method not specified. When 



utilizing urea as nitrogen source sterilize 

 the urea at 100°C. dry heat. 

 Use: Cultivation of albumin formers from 

 soil and stable manure. These organisms 

 grow very well on agar, gelatin and 

 bullion which contain 0.3% NaNOs. 

 The nitrate in this medium is changed to 

 nitrite after a few days and then quanti- 

 tatively to insoluble albuminous ma- 

 terials. Ammonia salts and urea also 

 are suitable nitrogen sources for albumin 

 production by these organisms. Albumin 

 production using urea, however, goes on 

 very slowly. 

 Added nutrients and variants : 



(a) The author employed the following 

 nitrogen sources: 



NaNOs (c.p. Merck) 3.0 g. 



(NH4)2S04 3.0 g. 



NH4NO3 2.0 g. 



NH4CO3 2.0 g. 



Urea 20.0 g. 



Cunningham and Lohnis used the follow- 

 ing basic solution to study the assimila- 

 tion of ammoniacal and nitrite nitrogen. 



1. Distilled water 1000.0 cc. 



2. Glycerol 10.0 g. 



3. K2HPO4 0.5 g. 



4. MgS04 0.02 g. 



5. NaCl 0.02 g. 



6. Fe2Cl6 trace 



Cunningham added 0.1%, of (NH4)2S04 or 



0.1% of NaNOi. 



