48 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Lohnis added 0.1% of urea sodium hip- 

 purate, uric acid, asparagin, ammonium 

 sulphate acetate, butyrate lactate or 

 NaNOs. Lohnis also specified that 

 other carbohydrates, alcohols and acids 

 might be used instead of glycerol. 

 Reference: Gerlach and Vogel (1901 p. 



612). Lohnis (1913 p. 94), Cunningham 



(1924 p. 153). 



146. 



Kuhne's Basal Lactate Solution 

 (Proskauer and Beck) 



Constituents : 



1. Water 600.0 cc. 



2. MgS04 3.5 g. 



3. Gypsum (burned) 1-5 g. 



4. NaCl 16.0 g. 



5. Magnesium (burned).. 2.5 g. . 



6. Potash (dried) 62.13 g. 



7. Soda 7.35 g. 



8. Ferrum reductum 6.2 g. 



9. H3PO4 (Sp. G. 1.3). .. 95.0 g. 



10. Lactic acid (Sp. G. 1.2). 50.0 to 60.0 g. 



11. Glycerol 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8, 9 and 10 in 1. 



(2) Add 12.0 cc. of (1), 40.0 g. glycerol, 

 5.0 g. NaCl and 1000.0 cc. of water 

 to one of the combinations listed 

 under added nutrients. 



Sterilization: Not specified. 



Use: To study the requirements for the 

 growth of tubercle bacilli. Growth gen- 

 erally occurred. The authors stated that 

 12.0 cc. of the basic solution was the 

 equivalent of 10.0 g. of commercial 

 Liebig's meat extract. 



Added nutrients: The authors use 12.0 cc. 

 of (1) basic salt solution and add 1000.0 

 cc. water, 40.0 g. glycerol and 5.0 g. of 

 NaCl. The following materials were 

 added to this solution: 



(a) tyrosine 10.0 g 



asparagin 2.0 g 



ammonium mucate 2.0 g 



taurine 0.5 g 



(b) leucine 4.0 g 



asparagin 2.0 g 



ammonium mucate 2.0 g 



taurine 0.5 g 



(c) tyrosine 1 -0 g 



ammonium mucate 2.0 g 



taurine 0.5 g 



(d) tyrosine 1 -0 g. 



asparagin 2.0 g. 



taurine 0.5 g. 



(e) tyrosine 10 g. 



asparagin 2.0 g. 



ammonium mucate 2.0 g. 



(f ) leucine 4.0 g. 



tyrosine 1 -0 g. 



asparagin 3.0 g. 



(g) leucine 4.0 g. 



tyrosine 1 -0 g. 



ammonium mucate 2.0 g. 



(h) leucine 4.0 g. 



asparagin 2.0 g. 



ammonium mucate 2.0 g. 



(i) tyrosine 1-0 g. 



asparagin 2.0 g. 



ammonium mucate 2.0 g. 



(j) leucine 4.0 g. 



tyrosine 1 g. 



taurine 0.5 g. 



(k) leucine 4.0 g. 



asparagin 2.0 g. 



taurine 0.5 g. 



(1) tyrosine 1-0 g. 



asparagin 2.0 g. 



taurine 0.5 g. 



(m) leucine 4.0 g. 



ammonium mucate 2.0 g. 



taurine 0.5 g. 



(n) tyrosine 1.0 g. 



ammonium mucate 2.0 g. 



taurine 0.5 g. 



(0) asparagin 2.0 g. 



ammonium mucate 2.0 g. 



taurine 0.5 g. 



(p) leucine 4.0 g. 



tyrosine 1 g. 



asparagin 2.0 g. 



ammonium mucate 2.0 g. 



taurine 0.5 g. 



(q) alanine 4.0 g. 



asparagin 2.0 g. 



(r) alanine 4.0 g. 



asparagin 3.0 g. 



ammonium mucate 3.0 g. 



(s) Used 7.35 soda instead of 6.35 g. in 



the basal solution and added: 



leucine 4.0 g. 



alanine 4.0 g. 



glycocoll 4.0 g. 



glucose 2.0 g. 



