CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



49 



(t) glycocoU 3.0 g. 



glycerol 40.0 g. 



taurine 5.0 g. 



(u) Tomasczewski used 7.35 soda in the 

 basal solution and added: 



tyrosine 1 -0 g 



leucine 4.0 g 



asparagin 2.0 g 



ammonium mucate 3.0 g 



taurine 0.5 g 



Reference: Proskauer and Beck (1894 p 



129-131, 134-135), Tomasczewski (1899 



p. 249). 



147. Calimard and Lacommes' Basal 



Glycerol Salt Solution (Kolle and 



Wassermann) 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl (0.5%) 5.0 g. 



3. MgS04 (0.05%) 0.5 g. 



4. Glycerol potassium phos- 

 phate (0.2%,) 2.0 g. 



5. Glycerol (1.5%,) 15.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and one 



of the added nutrients in 1. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Added nutrients: The author added one of 



the following: 



urea, amines, leucine, tyrosine, etc., etc. 

 Reference: Kolle and Wassermann (1912 p. 



394). 



148. Beijerinck's Modified Mannitol Salt 

 Solution (Omeliansky and Sswerowa) 



Constituents: 



1. Distilled water 



2. Mannitol 



3. Potassium phosphate.. 



4. MgS04 



5. NaCl 



6. Iron sulphate 



1000.0 cc. 

 20.0 g 

 1.0 g 

 0.5 g 

 0.1 g 

 0.1 g 

 Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not given. 

 Use: To study the pigment production of 



Azotobacter chroococcum. 

 Variants: Harvey used the following vari- 

 ant of this solution to study nitrification: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.2 g. 



4. NaCl 0.02 g. 



5. MnS04 (1:1000) 1.0 cc. 



6. FeS04 (1:1000) 1.0 cc. 



7. Mannitol 20.0 g. 



Reference: Omeliansky and Sswerowa 



(1911 p. 645), Harvey (1921-22 p. 105). 



149. Murray's Mannitol Salt Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.2 g. 



3. MgS04 0.2 g. 



4. NaCl 0.5 g. 



5. Mannite 20.0 g. 



6. FeCl. 1 drop of 10% sol. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Neutralize with KOH using phenol- 

 phthalein as indicator. 



Sterilization: Not specified. 



Use: To study the fixation of nitrogen 



under anaerobic and aerobic conditions 



by bacteria from the soil. 

 Reference: Murray (1916 p. 604). 



150. Percival's Mannitol Phosphate Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. KH,P04 2.0 g. 



3. Magnesium phosphate 10 g. 



4. Mannitol 20.0 g. 



Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Add 4 to (1). 



(3) Heat to boiling. 



(4) Filter. 



(5) Adjustment of reaction not specified. 



(6) Tube in 10 to 15.0 cc. quantities. 



(7) After sterilization inoculate with 

 contents of a nodule of a pea, runner 

 bean, red clover, or broad bean, by 

 means of a sterile knife. 



Sterilization: Sterilize on 3 successive 



days (time not given). 

 Use: Cultivation of bacteria from the roots 



of leguminous plants (bacteroids). 

 Reference: Percival (1920 p. 204). 



151. Capaldi and Proskauer's Basal Man- 



nitol Salt Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. NaCl (0.02%) 0.2 g. 



3. MgS04 (0.01%) 0.1 g. 



4. KH2PO4 (0.2%) 2.0 g. 



5. Mannitol (0.2%) 20.0 g. 



