50 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add a suitable nitrogen source. 

 Sterilization: Not specified. 



Use: To study acid production by B. colt. 

 Added nutrients : The authors added one of 



the following nitrogen sources: 



glycocoll 



alanine 



leucine 



oxamide 



succinamide 



tyrosine 



urea 



sulfocarbamid 



guanidinchlorhydrate 



guanine 



creatine 



sarcosine 



allantoin 



alloxantin 



alloxan 



theobromine 



caffein 



potassium urate 



glycocoll + urea 



ammonium formate 



ammonium acetate 



ammonium butyrate 



ammonium isobutyrate 



ammonium valerate 



ammonium stearate 



ammonium oleate 



ammonium lactate 



ammonium pyroracemate 



ammonium oxalate 



ammonium malonate 



ammonium succinate 



ammonium tartrate 



ammonium fumarate 



ammonium mucate 



ammonium citrate 



ammonium aconitate 



ammonium itaconate 



ammonium citraconate 



ammonium amidobenzoate 



ammonium hippurate 



ammonium orthonitrozimmtate 



ammonium piperate 



ammonium carbamate 



ammonium cyanurate 



ammonium carbonate 

 Reference: Capaldi and Proskauer (1S96 



p. 456). 



152. Percival's Succinate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KH2PO4 2.0 g. 



3. Magnesium phosphate 0.1 g. 



4. Sodium succinate 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 4 to (1). 



(3) Heat to boiling. 



(4) Filter. 



(5) Adjustment of reaction not specified. 



(6) Tube in 10 to 15.0 cc. quantities. 



(7) After sterilization inoculate with 

 contents of a nodule of a pea, runner 

 bean, red clover, or broad bean by 

 means of a sterile knife. 



Sterilization: Sterilize on 3 successive days 



(time not given). 

 Use: Cultivation of bacteria from the roots 



of leguminous plants, (bacteroids). 

 Reference: Percival (1920 p. 204). 



153. Winogradsky's Glucose Salt Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Glucose 20.0 g. 



3. Potassium phosphate .. . 1.0 g. 



4. MgS04 0.2 g. 



5. NaCl small amount 



6. FeS04 small amount 



7. MnS04 small amount 



8. Chalk 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Adjustment of reaction not specified. 



(3) Distribute into Drechsel's flasks with 

 aground cover. 



(4) Add an excess of chalk to each flask. 

 Sterilization: In the autoclave. 



Use : Used by Winogradsky to study nitro- 

 gen fixation by Clostridium Pastoriunum. 

 Other investigators used other nitrogen 

 fixing bacteria. 



Variants : 



(a) von Freudenreich used 20 to 40.0 g. 

 glucose, 0.5 g. of potassium phosphate 

 instead of 0.2 g., and specified 0.01 

 to 0.02 g. of NaCl, FeS04 and MnS04. 



(b) Jacobitz used the same variation as 

 (a) but specified distilled water. 



(c) Smith used 20 to 40.0 g. glucose, 

 0.5 g. MgS04, 0.01 to 0.02 g. NaCl, 

 0.1 to 0.2 g. iron sulphate, instead of 



