CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



51 



FeS04, omitted the MnS04 and used 

 30.0 to 40.0 g. CaCOs per liter. 



(d) Bredemann specified 1.0 g. K2HPO4, 

 0.02 g. NaCl, 0.1 g. FeS04, and 0.01 g. 

 MnS04 but did not specify distilled 

 water. 



(e) Percival specified 1.0 g. K..HP04, 

 used 30.0 g. glucose and 0.5 g. MgS04. 



(f) Lantzsch specified 1.0 g. K,HP04, 

 0.01 g. NaCl, 0.01 g. FeS04 and omit- 

 ted the MnS04. He did not specify 

 distilled water. 



(g) Lantzsch specified the use of 0.01 g. 

 NaCl, 0.2 g. FeS04 used 50.0 g. 

 CaCOa, and omitted the MnS04. 

 This solution was employed as a basic 

 solution and added one of the follow- 

 ing to each 100.0 cc. of solution: 



(a) 2.0, 5.0 or 10.0 cc. of a 2.0% NaOH 

 (soil extract). 



(b) 0.28 g. or 0.56 g. animal charcoal. 



(c) 0.4 g., 1.0 g. or 3.0 g. of Bolus alba 

 (pure kaolin). 



(d) 0.25% or 1.0% gelatin. 



This medium was used for the cultivation 

 of amylobacter spores. 

 References: Winogradsky (1902 p. 49), von 

 Freudenreich (1903 p. 516), Jacobitz 

 (1903 p. 100), Smith (1905 p. 199), Brede- 

 mann (1909 p. 496), Percival (1920 p. 174), 

 Lantzsch (1921 p. 2), Lantzsch (1921 p. 5). 



154. Stutzer, Burri and Maul's Glucose 

 Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asbestos 



3. Potassium phosphate 10 g. 



4. MgS04 0.5 g. 



5. NaCl 1.0 g. 



6. CaCU 0.1 g. 



7. Dextrose 20.0 g. 



Preparation : 



(1) Dissolve 3, 4, 5, 6 and 7 in 1. 



(2) Heat long fibre asbestos to glowin?. 



(3) To a 2 liter flask add 10.0 g. (2) 25.0 

 cc. (1), 25.0 cc. sterile water. 



(4) After sterilization add a large plati- 

 num loop of a gelatin streak culture 

 of an organism to 100.0 cc. sterile 

 water. Add 2.0 cc. of (5) to (4). 



Sterilization: Method not given. 

 Use: To determine nitrogen fixation. 

 Authors used the bacteria from mustard 



and alfalfa plant nodules and found that 

 little, if any, nitrogen was assimilated 

 after 60 days incubation at 28 to 30'^C. 

 Reference: Stutzer, Burri and Maul (1896 

 p. 669). 



155. Stoklasa's Basal Salt Solution 



Constituents : 



1. Water 2300.0 cc 



2. Sodium phosphate 0.5 g 



3. K.SO4 0.2 g. 



4. MgClo 0.05 g. 



5. Glucose 2.5 g. 



6. CaCOs 10.0 g. 



Preparation : 



(1) Dissolve 0.5 g. sodium phosphate, 

 0.2 g. potassium sulphate and 0.05 g. 

 magnesium chloride in 500.0 cc. of 

 water. 



(2) Dissolve 2.5 g. glucose in 500.0 cc. 

 water. 



(3) Mix 200.0 cc. (1) with 100.0 cc. (2). 



(4) Dissolve 6 and one of the added 

 nutrients in (3). 



(5) Dilute to 20C0.0 cc. 

 Sterilization: Method not given. 



Use: To study utilization of nitrogen by 

 B icillus megatherium, {Bacillus Ellen- 

 bichii) or "Alinit." The author reported 

 that more nitrogen was found to be in 

 solution after incubation. These organ- 

 isms decomposed nitrogenous material to 

 a form that may be absorbed by the root 

 hairs. 



Added nutrients: The author added 50.0 g. 

 of glutin or fibrin. 



Reference: Stoklasa (1898 p. 508). 



156. Charpentier's Glucose Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.25 g. 



3. KH2PO4 0.25 g. 



4. MgS04 0.37 g. 



5. Iron phosphate trace 



6. CaS04 trace 



7. NaCl 0.2 g. 



8. Glucose 

 Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in flasks. 



(3) Add 0.075 g. glucose to each flask. 

 Amount of medium in the flask not 

 specified. 



