52 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(4) Pass a current of air thru H2SO4 and 

 then thru the culture. 

 Sterilization: Not specified. 

 Use: Nitrogen fixation by algae; Cysto- 



coccus humicola did not fix free nitrogen. 

 Reference: Charpentier (1903 p. 322). 



157. Krzemieniewska's Glucose Salt 

 Solution (Vogel) 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04-7H20 0.25 g. 



3. CaHP04 0.25 g. 



4. Glucose 13.5 g. 



5. K2SO4 0.0 to 0.872 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add from 0.0 to 87.2 mg. of K2SO4 

 to(l). 



Sterilization: Not specified. 



Use: Nitrogen fixation. The author found 



that the most nitrogen was fixed in the 



presence of 0.872 g. K2SO4. 

 Reference: Vogel (1911-12 p. 417). 



158. Kriiger and Schneidewind's Basal 

 Glucose Salt Solution (Heinze) 



Constituents : 



1. Water 1000.0 cc. 



2. Glucose (1.0%) 10.0 g. 



3. K3PO4 (0.2%) 2.0 g. 



4. MgS04 (0.04%) 0.4 g. 



5. CaCl2 (0.02%) 0.2 g. 



6. FeCh (20.0% soln.) 1 drop 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the nitrogen sources listed 

 in added nutrients. 



Sterilization: Not specified. 



Use: To study nitrogen assimilation by 

 green algae, Chlorella, Chlorothecimn, 

 Stichococcus . The author reported that 

 nitrogen was not assimilated. Poor 

 growth unless nitrogen was present. 



Variants: The solution may be used with- 

 out the addition of any nitrogen source. 



Added nutrients: The author added 0.25% 

 of (NH4)2S04or NaNOs. 



Reference: Heinze (1906 p. 647). 



159. Charpentier's Basal Glucose Salt 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04 1.0 g. 



3. K2HPO4 2.0 g. 



4. CaCl2 0.1 g. 



5. FeS04 0.05 g. 



6. Glucose 10.0 g. 



Preparation : 



(1) Dissolve one of the added nutrients 

 in water. 



(2) Dissolve 2, 3, 4, 5 and 6 in the re- 

 mainder of the water. 



(3) Mix sterile (1) and (2) under aseptic 

 conditions. 



Sterilization: Sterilize (1) by filtration and 

 (2) in the autoclave. 



Use: To study assimilation of nitrogen by 

 algae, Cystococcus humicola. Author 

 reported that Ca(N03)2 was a more 

 suitable nitrogen source than asparagin 

 or peptone. 



Added nutrients and variants: 



(a) The author added 1.8 g. of peptone or 

 1.8 g. asparagin as a nitrogen source. 



(b) Miiller omitted the FeS04, used 0.1% 

 K2HPO4 and 0.2% MgS04 in the 

 basic solution and added one of the 

 following nitrogen sources. He culti- 

 vated Bacterium Giintheri. 



KNO3 



ammonium tartrate 

 aspn-ragin 

 peptone 

 References: Charpentier (1903 p. 333), 

 Miiller (1907 p. 472). 



160. Stoklasa's Basal Glucose Salt Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. d-glucose 25.0 g. 



3. Phosphoric acid anhydride. 0.2 g. 



4. K2SO4 1.0 g. 



5. MgCl2 0.5 g. 



6. Iron sulphate + 



7. Aluminum sulphate 0.1 g. 



8. CaCl2 0.1 g. 



Preparation : 



(1) Dissolve 2, 4, 5, 6, 7 and 8 in 1. 



(2) Add one of the added nutrients to (1). 



(3) Add 0.2 g. of phosphoric acid anhy- 

 dride in the form of monomagnesium 

 phosphate, monodialuminum phos- 

 phate, monodiferric phosphate or 

 trimagnesium phosphate to (2). 



(4) Inoculate 8 days following steri- 

 lization. 



Sterlization : In the autoclave. 



Use: To study role of phosphorus in the 



