CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



53 



soil . Development of the bacteria hardly 



visible when phosphoric anhydride was 



omitted. 

 Added nutrients: The author added 1.0 g. 



nitrogen in the form of (NH4)2S04 or 



(NH4)N03. 

 Reference: Stoklasa (1911 p. 490). 



161. Henneberg's Basal Glucose Salt 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KH2PO4 (0.1%) 1.0 g. 



3. MgS04 (0.02%) 0.2 g. 



4. CaCl2 (0.01%) 0.1 g. 



5. Glucose (2.0%) 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 1.0% 



of one of the added nutrients in 1. 

 Sterilization: Not specified. 

 Use: To study nitrogen requirements for 



lactic acid bacteria. Urea, asparagin or 



ammonium phosphate were found not to 



be suitable as nitrogen sources. 

 Added nutrients: The author added 1.0% 



of one of the following: 



peptone asparagin 



casein urea 



gluten ammonium phosphate 



Reference: Henneberg (1903 p. 7). 



162. Gottheil's Carbohydrate Glycerol 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1-0 g. 



3. CaCl2 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



6. Iron trace 



7. Dextrose 5.0 g. 



8. Cane sugar 5.0 g. 



9. Glycerin 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8 



and 9 in 1. 

 Sterilization: Not specified. 

 Use: Used as a general culture medium. 



Author used bacteria found in the soil 



and on the roots of plants. 

 Reference: Gottheil (1901 p. 432). 



163. Harvey's Citric Acid Glucose Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Glucose 2.0 g. 



3. Citric acid 5.0 g. 



4. MgS04 2.0 g. 



5. K2HPO4 2.0 g. 



6. CaCl2 0.2 g. 



7. FeCla trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in distilled 

 water. 



(2) Reaction should be neutral (indicator 

 not specified). 



Sterilization: Not given. 

 Use: Nitrogen fixation by aerobic nitro- 

 gen fixing bacteria. 

 Reference: Harvey (1921-22 p. 104). 



164. Miinter's Basal Mannitol Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Mannitol 2.0 g. 



3. Glucose 10.0 g. 



4. MgS04 0.5 g. 



5. NaCl 0.5 g. 



6. K2HPO4 2.0 g. 



7. CaCl2 0.1 g. 



8. FeCls trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Add 0.02 or 0.1 g. of nitrogen in the 

 form of one of the added nutrients. 



(3) Adjustment of reaction not given. 



(4) Distribute in 100.0 cc. lots. 

 Sterilization: Not specified. 



Use: To study availability of nitrogen for 

 Actinomycetes odorifer, Act. chromo- 

 genes, Act. albus. NH4CI, NH4NO3 and 

 asparagin were found to be equally suit- 

 able nitrogen sources for the actinomy- 

 cetes studied. 



Added nutrients: The author used the 

 following nitrogen sources: 

 NH4CI 

 NH4NO3 

 asparagin 



Reference: Miinter (1913 p. 368). 



165. Chrzaszcz's Basal Sucrose Salt 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. MgS04 2.0 g. 



4. K2SO4 2.0 g. 



5. Na2HP04 5.0 g. 



6. CaCOa 1.0 g. 



