54 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the added nutrients to (1). 

 Sterilization: Not specified. 



Use: To study growth requirements of 

 yeasts. Author reported that yeast grew 

 more luxuriantly in a medium containing 

 peptone than asparagin. Growth best, 

 however, in sugar beer wort medium. 



Added nutrients: The author added 5.0 g. 

 of asparagin or 5.0 g. of peptone. 



Reference: Chrzaszcz (1904 p. 148). 



166. Maze's Sucrose Salt Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Sucrose 20.0 g. 



3. Potassium phosphate 10 g. 



4. NaCl 1.0 g. 



5. FeS04 trace 



6. MgS04 trace 



7. ZnCl2 trace 



8. Calcium saccharate trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Adjustment of reaction not specified. 

 Sterilization: Sterilize at 120°C. (time not 



given). 

 Use: Cultivation of bacteria from the 



nodules of leguminous plants, nitrogen 



fixing bacteria. 

 Reference: Maze (1898 p. 12). 



167. Linde's Basal Sucrose Salt Solution 



Constituents : 



1. Distilled water 



2. K2HPO4 



3. MgS04-7H20 



4. NaCl 



5. CaCl2 



6. Sucrose (0.2%) 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add 0.15% of one of the nitrogen 

 sources given in added nutrients. 



Sterilization: Not specified. 



Use: Cultivation of Cladothrix. The 



author reported that the organism grew 



better on meat extract media. 

 Added nutrients: The author added 0.15% 



of one of the following: 



KNO3 (NH4)3P04 



NH4CI (NH4)2S04 



Reference: Linde (1913 p. 387). 



168. Lohnis and Lochhead's Basal Cellulose 



Salt Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Basic slag ("Thomasmehl") 

 (0.2%) 2.0 g. 



3. K2HPO4 (0.02%) 0.2 g. 



4. MgS04 (0.01%) 0.1 g. 



5. NaCl (0.001%) 0.01 g. 



6. Filter paper. 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the nitrogen sources 

 listed under added nutrients. 



(3) The reaction is slightly alkaline, but 

 may be neutralized by HCl or lactic 

 acid. 



(4) Place strips of filter paper in flasks. 



(5) Pour about 10.0 cc. of (2) over the 

 paper. 



(6) Inoculate with about 1.0 g. of soil 

 or cow manure. 



Sterilization: Not specified. 



Use: Cultivation of cellulose dissolving 

 bacteria. Author found that the best 

 growth was obtained on medium con- 

 taining 0.1% beef extract. KNO3 was 

 the best inorganic nitrogen source. The 

 solution was carefully poured from the 

 filter paper as soon as it has become turbid 

 by bacterial growth and fresh solution 

 added. 



Added nutrients: The author used the 

 following nitrogen sources in the amounts 

 indicated: 



Peptone 0.1 or 0.02% 



Beef extract 0.1 or 0.02% 



Asparagin 0.25 or 0.05% 



(NH4)2S04 0.5 or 0.1% 



NH4NO3 0.25 or 0.05% 



KNO3 0.5 or 0.1% 



Reference: Lohnis and Lochhead (1923 

 p. 431). 



169. van Iterson's Basal Cellulose Salt 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Paper 20.0 g. 



3. K2HPO4 0.5 g. 



Preparation : 



(1) Mixl, 2 and 3. 



(2) Add one of the nitrogen sources given 

 in added nutrients, to (1). 



