CULTUEE MEDIA FOR CULTIVATION OF MICROORGANISMS 



55 



(3) When using KNO3 distribute by 

 filling 200.0 cc. flasks. When using 

 other nitrogen sources distribute in 

 thin layers in flasks. 



(4) Inoculate with canal water contain- 

 ing some mold or decay (moder). 



Sterilization: Not specified. 



Use: To study the decomposition of cellu- 

 lose by denitrifying organisms. The 

 author reported denitrification and de- 

 composition of cellulose with the pro- 

 duction of N2 and CO2. 



Added nutrients: The author used the 

 following nitrogen sources: 



KNO3 2.5 g. 



NH4CI 1.0 g. 



KNO2 amount not given 

 MgNH4P04 amount not given 

 peptone amount not given 



Variants : The author sprinkled MgNH4P04 

 between two Swedish filter papers and 

 placed them in a flask. Then he poured 

 a solution of 0.5 g. K2HPO4 in a liter of 

 water over the paper. He found that 

 Bacillus f err ugineus from the soil decom- 

 posed the cellulose. 



Reference: van Iterson (1903-4 p. 690). 



170. Jones' Modified Ashby's Mannitol 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Mannitol 20.0 g. 



3. IV2HPO4 0.2 g. 



4. MgS04 0.2 g. 



5. NaCl 0.2 g. 



6. CaS04 0.1 g. 



7. CaCh 5.0 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Azotobacter. 

 Variants : 



(a) Bonazzi specified the use of tap 

 water, used 0.408 g. MgS04-7H20; 

 0.127 g. CaS04; 0.2 g. CaCOs and 

 specified that Ca(N03)2 might be 

 added if desired (amount not given). 



(b) Giltner used 15.0 g. of mannitol and 

 added 1 drop of 10.0% Fe2Cl6 solution. 



Reference: Jones (1913 p. 14), Bonazzi 

 (1921 p. 357), Giltner (1921 p. 376). 



171. Fred and Loomis' Mannitol Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Mannitol 10.0 g. 



3. MgS04 0.2 g. 



4. KH2PO4 0.2 g. 



5. NaCl 0.2 g. 



6. CaS04 0.1 g. 



Preparation : 



(1) Dissolve 2, 3, 5 and 6 in 1. 



(2) Dissolve phosphate separately in a 

 little water and add to (1). 



(3) Make (2) neutral to phenolphthalein 

 with N/1 NaOH. 



(4) After sterilization add NaOH and 

 H2SO4 to obtain pH value from 2.7 

 to 11.1. 



Sterilization: Method not specified. 



Use: To show the effect of hydrogen ion 

 concentration on growth of Rhizobium. 

 Maximum growth at pH 7.2. None in 

 pH 2.77 and 3.1. Yamagata and Itano 

 found the optimum pH for azotobacter 

 to be between 6.6 and 7.7 depending on 

 the type of azotobacter. 



Variants: Yamagata and Itano used 20.0 g. 

 of mannitol instead of 10.0 g. 



Reference: Fred and Loomis (1917 p. 629), 

 Yamagata and Itano (1923 p. 522). 



172. Krzemieniewska's Mannitol Salt 



Solution (Vogel) 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 2.5 g. 



3. MgS04 2.5 g. 



4. CaS04 2.5 g. 



5. Mannitol 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Azotobacter. 



Variants: The author used 2.5 g. CaHPOi 

 instead of 2.5 g. CaS04, or omitted the 

 K2HPO4 and added 2.5 g. CaHPO* instead 

 of CaS04. 



Reference: Vogel (1911-12 p. 416). 



173. Harvey's Mannitol Salt Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Mannitol 15.0 g. 



3. MgS04 0.2 g. 



4. KH2PO4 0.2 g. 



