CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



57 



lysine dichloride 

 taurine 



Variants: The authors found that com- 

 binations of amino acids possessed little 

 value over single amino acids. 



Reference : Koser and Rettger (1919 p. 304), 

 Koser (1918, p. 12). 



177. Puriewitsch's Tartaric Acid Salt 



Solution (Heinze) 

 Constituents : 



1. Water 1000.0 cc 



2. Tartaric acid 3.0 g. 



3. MgS04 0.2 g. 



4. CaCli 0.4 g. 



5. KH2PO4 0.4 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Study of nitrogen assimilation by 

 molds, Aspergillus niger and Penicillum 

 glaucum. 



Reference: Puriewitsch (1895 p. 342 from 

 an abstract by Heinze 1903 p. 27). 



178. Omeliansky's Cellulose Solution 

 Constituents: 



1. Water 



2. Chalk 



3. Paper. 



Preparation: (1) Inoculate a flask of water 

 containing chalk and paper with some 

 river mud. 



Use: To study cellulose fermentation by 

 organisms found in mud of rivers. The 

 paper was completely destroyed after 

 3.5 years. 



Reference: Omeliansky (190 p. 229). 



179. Killer's Mannitol Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Mannitol 20.0 g. 



3. Potassium phosphate 0.5 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: To study nitrogen assimilation by 



soil bacteria and protozoa. 

 Variants : Dilute the medium 5 to 10 times 



for cultivation of protozoa. 

 Reference: Killer (1913 p. 522). 



180. Ringer's Salt Solution (Park, Williams 



and Krumwiede) 

 Constituents : 



1. Water 1000.0 cc. 



2. NaCl 10.0 g. 



3. KCl 0.2 g. 



4. CaCl2 0.2 g. 



5. Sodium bicarbonate 0.1 g. 



6. Glucose 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Diluent and a basis solution to which 



carbonaceous and nitrogenous material 



may be added. (See medium 600.) 

 Reference: Park, Williams and Krumwiede 



(1924 p. 122). 



181. Beijerinck's Glucose Salt Solution 

 Constituents: 



1. Water (ditch) 1000.0 cc. 



2. Glucose 0.05 g. 



3. Potassium phosphate 0.1 g. 



4. FeCls several drops 



5. NasCOs 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in ditch water 

 ("Grabenwasser") containing 45 mg. 

 SO3 per liter. 



(2) Distribute into flasks or beaker. 

 Cover the beaker with a glass plate 

 or fill the flask full to reduce the sur- 

 face exposed to the air. 



Sterilization: Not specified. 



Use: To study sulphate reduction by 

 Spirillum, desulfuricans. US and H2SO3 

 can be detected after 12 hours. 



Reference: Beijerinck (1895 p. 57). 



182. Stoklasa's Glucose Salt Solution 

 Constituents: 



1. River water 1000.0 cc. 



2. Glucose 20.0 g. 



3. K2HPO4 0.5 g. 



4. NaoCOa 0.25 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in thin layers in large 

 flasks. 



Sterilization: Sterilize thoroughly in the 

 autoclave. 



Use: To study the utilization of carbo- 

 hydrate and nitrogen assimilation by 

 Azotobacter chroococcum Beijerinck. 



Reference: Stoklasa (1908 p. 503). 



183. Gage's Maltose Salt Solution 

 Constituents: 



1. Water nitrite free 1000.0 cc. 



2. KH2PO4 1.0 g. 



