CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



59 



extract instead of water in variant 

 (a). Nitrogen assimilation was bet- 

 ter in plain solution however, 

 (c) Hanzawa suggested the addition of 

 humus or saltpeter in varying 

 amounts to variant (a). 

 References: Wojtkiewicz (1914 p. 255), 

 Hanzawa (1914 p. 574). 



188. Krainsky's Mannitol Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 0.5 g. 



3. NaCl 0.5 g. 



4. CaCOa 0.5 g. 



5. FeSOj trace 



6. Mannite 10.0 g. to 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Enrichment of Azotobader chro- 



ococcum. 

 Reference: Krainsky (1908 p. 726). 



189. Peklo's Modified Beijerinck's Man- 



nitol Salt Solution 



Constituents: 



1. Water (river) 1000.0 cc. 



2. Mannitol 25.0 g. 



3. K^HPOi 8.0 g. 



4. K2CO3 4.0 g. 



5. CaCOa 

 Preparation : 



(1) Dissolve 2, 3 and 4 in Moldau river 

 water. 



(2) Add CaCOa, amount not specified. 

 Sterilization: Not specified. 



Use: Cultivation of plant actinomyces. 

 Reference: Peklo (1910 p. 514). 



190. Butcher's Basal Chinate Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. Calcium chinate 10.0 g. 



4. FeCls 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4 and one of 



the added nutrients in 1. 



Sterilization: Not specified. 



Use: Differentiation of colon-aerogenes 

 group. Author reported that a solid 

 medium was preferred to a liquid medium. 

 Bad. aerogenes gave a black coloration 

 to the medium, while Bad. coli did not. 



Added nutrients: The author added 0.5 g. 



NH4CI or 0.5 g. peptone. 

 Reference: Butcher (1924 p. 295). 



SUBGROUP I-C. SECTION 2 



Liquid media or basal solutions with con- 

 stituents of known chemical composition; 

 nitrogen supplied as ammonium salts, 

 carbon organic. 

 Ai.* Media primarily for growth of yeast, 



fungi, or algae; not bacteria. 

 Bi. Ammonia supplied as ammonium 

 chloride. 

 Wilder's Sugar Salt Solution 



(Chrzaszcz) 19I 



LaGarde's Sucrose Ammonium Chlo- 

 ride Solution 192 



Fulmer and Nelson's Sucrose Am- 

 monium Chloride Solutions 193 



Bj.t Ammonia Supplied as ammonium ni- 

 trate. 

 Ci. Organic carbon supplied only as carbo- 

 hydrates. 

 Di. Mono or disaccharides added. 

 El. Calcium salts added. 

 Mayer's Sucrose Ammonium Nitrate 



Solution (Pringsheim) 194 



Bokorny's Sucrose Ammonium Ni- 

 trate Solution 195 



E2. Calcium salts not added. 

 Hollborn's Sucrose Ammonium Ni- 

 trate Solution 196 



Laborde's Sucrose Ammonium Ni- 

 trate Solution 197 



Ono's Sucrose Ammonium Nitrate 



Solution 198 



D2. Polysaccharides added. 

 Behren's Cellulose Ammonium Ni- 

 trate Solution 199 



C2. Organic carbon not supplied exclusively 

 as carbohydrates. 



Raulin's Solution (Smith) 200 



Behrens' Tartrate Ammonium Ni- 

 trate Solution 201 



Behrens' Citrate Ammonium Nitrate 



Solution 202 



von Tubeuf's Cellulose Ammonium 



Nitrate Solution 203 



B3. Ammonia supplied as ammonium sul- 

 phate. 



*See next page for A2. 

 tSee B3, B4, Bs, Be and B7 



