CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



63 



Proskauer and Beck's Basal Glycerol 

 Ammonium Tartrate Solution 319 



Uschinsky's Glycerol Ammonium Lac- 

 tate Solution 320 



Fermi and Montesano's Glycerol Am- 

 monium Tartrate Solution 321 



D2. Containing more than one additional 



carbon source. 



Gottheil's Ammonium Tartrate Solu- 

 tion. No. VI 322 



MacKensie's Ammonium Tartrate 

 Solution (Smith) 323 



191. Wilder's Sugar Salt Solution 

 (Chrzaszcz) 



Constituents: 



1. Water 1000.0 cc. 



2. Sugar 100.0 g. 



3. MgS04 2.5 g. 



4. KCl 2.5 g. 



5. NH4CI 2.5 g. 



6. Na2HP04 2.5 g. 



7. CaCOa 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) A white precipitate of Ca3P04 forms 

 when CaCO'; is added. This may or 

 may not be filtered out. 



(3) Distribute in flasks or tubes. 

 Sterilization: Not specified. 



Use: To study growth requirements of 

 yeasts. The filtered medium showed less 

 growth of yeast than the non-filtered 

 material. 



Reference: Chrzaszcz (1904 p. 145). 



192. LaGarde's Sucrose Ammonium Chlo- 

 ride Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Cane sugar 30.0 g. 



3. NH4CI 6.0 g. 



4. MgS04 0.5 g. 



5. KH2PO4 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Phycomyces nilens, 

 Mucor Mucedo, Mucor Rouxii, Mucor 

 corymbifer, Mucor spinosus, Mucor race- 

 mosus, Mucor rhizopodiformis, Mucor 

 stolonifer. 



Reference: LaGarde (1911-12 p. 248). 



193. Fulmer and Nelson's Sucrose Ammo- 



nium Chloride Solutions 



Constituents : 



1. Water 1000.0 cc. 



2. NH4CI 1.88 g. 



3. Sucrose 100.0 g. 



4. K2HPO4 1.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjustment of reaction not given. 

 Sterilization: Method not specified. 



Use: Continuous cultivation of Saccharo- 

 myces cerevisiae. Authors found that 

 these solutions gave continuous growth. 



Variants : 



(a) Authors added 1.0 g. CaCl2 to above 

 solution. 



(b) Authors added 1.0 g. CaClo and 6.0 g. 

 dextrin to the above solution. 



Reference: Fulmer and Nelson (1923 p. 

 132). 



194. Mayer's Sucrose Ammonium Nitrate 



Solution (Pringsheim) 



Constituents : 



1. Water 1000.0 cc. 



2. Sugar 100.0 g. 



3. KH2PO4 5.0 g. 



4. MgS04 5.0 g. 



5. Ca3(P04)2 0.5 g. 



6. NH4NO3 7.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization : Not specified. 

 Use: To study bios requirements of yeast. 



Growth and fermentation occurred after 



one day. 

 Reference: Pringsheim (1906 p. 114). 



195. Bokomy's Sucrose Ammonium Nitrate 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NH4NO3 (0.47%) 4.7 g. 



3. rNH4)2S04 (0.005%) 0.05g. 



4. Cane sugar (9.0%) 90.0 g. 



5. Dipotassium phosphate 

 (0.035%) 0.35 g. 



6. MgS04 (0.036%) 0.36 g. 



7. CaCl2 (0.0015%) 0.015 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization : Not specified. 



