64 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: To study yeast growth. 

 Reference: Bokorny (1911 p. 182). 



196. HoUbom's Sucrose Ammonium Nitrate 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Sugar 50.0 g. 



3. (NHONOs 10.0 g. 



4. KH2PO4 5.0 g. 



5. MgS04 2.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Tricophyton rodens 

 causing "Alopecia areata," one kind of 

 baldness. Growth similar to that in 

 bouillon. Growth on the surface — first 

 olive green and later darker green. 

 Similar medium used to study fermenta- 

 tion by Aspergillus niger. 



Variants: Currie recommended the use of 

 the following medium to study citric acid 

 fermentation of Aspergillus niger: 



1. Water 1000.0 cc. 



2. Sucrose 125.0-150.0 g. 



3. NH4NO3 2.0-2.5 g. 



4. KH2PO4 0.75-1.0 g. 



5. MgS04-7H20 0.2-0.25 g. 



6. HCl to give pH between 3.4^3.5 (4-5 

 cc. N/5 HCl). 



197. Laborde's Sucrose Ammonium Nitrate 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Sugar 50.0 g. 



3. NH4NO3 2.0 g. 



4. Potassium phosphate 1.0 g. 



5. MgS04 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization : Not specified. 



Use: Cultivation of the mold Enrotiopsis 



Gayoni. 

 Reference: Laborde (1897 p. 13). 



198. Ono's Sucrose Ammonium Nitrate 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Cane sugar 85.0 g 



3. NH4NO3 16.0 g 



4. MgS04 3.0 g 



4. KCl 3.0 g 



6. NH4H2PO4 30 g 



7. FeS04 trace 



8. CUSO4 solution 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in 30.0 cc. lots. 



(3) Add CUSO4 so that the flasks will 

 contain from 1/250 to 1/32,000 Mol 

 CUSO4. 



Sterilization : Sterilize in the steamer. 



Use: To study the effect of small amounts 

 of CuS04 on the growth of Aspergillus 

 niger. It was found that small amounts 

 of CUSO4 tend to increase growth. 

 Higher concentration than 1/250 tend to 

 hinder growth. 



Reference: Ono (1902 p. 155). 



199. Behrens' Cellulose Ammonium Nitrate 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NH4NO3 10.0 g. 



3. Potassium phosphate 5.0 g. 



4. MgS04 2.5 g. 



5. Filter paper (Swedish) 50.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) After sterilization add 5.0 g. of puri- 

 fied Swedish filter paper containing 

 some of the mycelium. 



Sterilization : Method not given. 



Use: Cultivation of Pseudo-Demaiophora. 



Growth was lu.xuriant. Utilized cellulose, 



as a source of carbon. 

 Reference: Behrens, (1897 p. 640). 



200. Raulins Solution (Smith) 



Constituents : 



1. Water 1500.0 cc 



2. Sugar 70.0 g 



3. Tartaric acid 4.0 g 



4. NH4NO3 4.0 g 



5. Ammonium Phosphate 0.6 g 



6. K2CO3 0.6 g 



7. MgS04 0.4 g 



8. (NH4)3S04 0.25 g 



9. ZnS04 0.07 g, 



10. FeS04 0.07 g, 



11. KSiOs 0.07 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8, 



9, 10 and 11 in 1. 

 Sterilization : Not specified. 

 Use: Cultivation of Aspergillus niger and 

 other molds. 



