CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



65 



Variants : 



(a) Bezangon did not specify the type 

 of ammonium phosphate, and speci- 

 fied that iron sulphate was to be used. 



(b) Harvey omitted the MgCOs and did 

 not specify the type of ammonium 

 phosphate. 



(c) Dopter and Sacquepee, and Heine- 

 mann, did not specify the type of 

 ammonium phosphate. 



(d) Tanner omitted the K2CO3. 



(e) Harvey also gave the following 

 solution: 



1. Distilled water 1500.0 cc. 



2. NH4NO3 4.5 g. 



3. Sucrose 70.0 g. 



4. Potassium tartrate 6.5 g. 



5. K2HPO4 0.6 g. 



6. MgCOs 0.4 g. 



7. K2SO4 0.25 g. 



8. FeS04 0.07 g. 



9. ZnS04 0.07 g. 



10. KaSiOa 0.07 g. 



(f ) Betrand and Javillier gave the follow- 

 ing modification: 



1. Water 1000.0 cc. 



2. Sugar 46.6 g. 



3. Tartaric acid 2.66 g. 



4. K2CO3 0.4 g. 



5. NH4NO3 2.66 g. 



6. Ammonium phosphate. . 0.4 g. 



7. (NH4)2S04 0.155 g. 



8. MgS04 0.71 g. 



9. Iron aluminum 0.081 g. 



10. K2Si03 0.046 g. 



(g) Roux and Rochaix did not specify 

 the type of ammonium phosphate 

 used, used 0.4 g. MgCOs instead of 

 MgS04, and 0.07 g. K2SO4 instead of 



K2Si03. 



References: Smith (1905 p. 197), Roux and 

 Rochaix (1911 p. 104), Brenner (1914 

 p. 556), Bezangon (1920 p. 20), Besson 

 (1920 p. 36) Harvey (1921-22 p. 103), 

 Dopter and Sacquepee (1921 p. 16), Tan- 

 ner (1919 p. 66), Heinemann (1922 p. 42), 

 Betrand and Javillier (1912 p. 244). 



201. Behrens' Tartrate Ammonium Nitrate 

 Solution. 



Constituents : 



1. Water 1000.0 cc. 



2. Sucrose 50.0 g. 



3. Tartaric acid 5.0 g. 



4. NH4NO3 . 5.0 g. 



5. K2HPO4 2.0 g. 



6. MgS04 1.0 g. 



7. Tannin 0.0, 1:1000, 1:500, 1:100 or 

 1:50. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute in 50.0 cc. lots. 

 Sterilization: Method not given. 



Use: Cultivation of Botrytis. Tannin in- 

 hibits growth, 0.5 and 1.0 g. per flask 

 showed no growth of the spores. 



Variants: The author added 0.0, 0.05 g. 

 (1:1000), 0.1 g. (1:500), 0.5 g. (1:100) or 

 1.0 g. (1:50) of tannin to each flask before 

 sterilization. 



Reference: Behrens' (1898 p. 642). 



202. Behrens' Citrate Ammonium Nitrate 

 Solution. 



Constituents : 



1. Water 1000.0 cc. 



2. Cane sugar 100.0 g. 



3. NH4XO3 20.0 g. 



4. Citric Acid 10.0 g. 



5. Potassium phosphate 10.0 g. 



6. MgSOi 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1 liter of 

 water rich in potassium. 



(2) Distribute in 50.0 cc. lots in 300.0 cc. 

 flasks (28 flasks). 



Sterilization: Method not given. 



Use: To determine influence of chemicals 

 on growth of Pseudodetnatephaca. 

 Growth takes place in copper sulphate 

 1:100,000, 1:10,000 and 1:5000. In iron 

 sulphate 1:100,000 to 1:500; in sodium 

 fluoride 1 : 100,000, and in potassium 

 sulfocarbonate 1:100,000 to 1:500. 



Added materials: The author added the 

 following materials: 



(a) 1.0 and 0.1% solutions of copper 

 vitrol, iron vitrol or sodium fluoride 

 so that the concentration would be 

 1:100, 1:200, 1:500, 1:1000, 1:5000, 

 1:10,000, 1:100,000 (previous to ster- 

 ilization). 



(b) 1.0% and 0.1% sterile solutions of 

 potassium sulphocarbonate so that 

 the concentration would be 1:100, 

 1:200, 1:500, 1:1000, 1:5000, 1:10,000, 

 1:100,000 (added to the sterilized 

 medium under aseptic conditions). 



Reference: Behrens' (1897 p. 745). 



