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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



203. von Tubeuf s Cellulose Ammonium 

 Nitrate Solution. 



Constituents : 



1. Water 1000.0 cc. 



2. NH4NO3 10.0 g. 



3. Potassium phosphate 5.0 g. 



4. MgS04 1.0 g. 



5. Lactic acid 2.0 g. 



6. Cellulose 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 50.0 cc. lots. 



(3) Prepare a series of flasks by adding 

 10.0 g. filter paper (Swedish) or 10.0 g. 

 ordinary filter paper, or 10.0 g. of 

 pine shavings or 10.0 g. of wadding 

 (10.0 g. sucrose with 5.0 g. gelatin 

 were also added). 



Sterilization: Not specified. 



Use: Cultivation of Merulius lacrymans 

 (causing dry rot). Good growth using 

 Swedish filter paper as carbon source. 

 Little growth on shavings or wadding. 

 Good growth on ordinary malt and meat 

 extract containing cane sugar and citric 

 acid 1 to 3%. 



Reference: v Tubeuf (1902 p. 130). 



204. Charpentier's Glucose Ammonium 



Sulphate Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Glucose 10.0 g. 



3. FeS04 trace 



4. (NH4)2S04 0.5 g. 



5. CaCl2 0.023 g. 



6. K2HPO4 2.0 g. 



7. MgS04 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in 100.0 cc. lots. 

 Sterilization: Not specified. 



Use : Nitrogen assimilation by Algae, Cys- 



tococcus humicola. 

 Reference: Charpentier (1903 p. 330). 



205. Artari's Syrup Ammonium Sulphate 



Solution (Owen) 

 Constituents: 



1. Water 1000.0 cc 



2. NasHPOi (0.15%) 0.15 g 



3. MgS04 (0.1%) 0.1 g 



4. (NH4)2S04 (0.15%) 0.15 g 



5. Grape sugar (2.0%) 2.0 g 



6. Cane syrup 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. This is 

 Artari's solution. 



(2) Prepare the following modifications 

 with cane syrup. Preparation of 

 syrup or exact composition not 

 given (may be 50.0% cane sugar 

 solution). 



(a) Make no additions to cane syrup. 



(b) To 2 parts cane syrup, add 1 part 



(c) Add an equal amount of (1) to cane 

 syrup. 



(d) To 1 part cane syrup add 2 parts 

 (1). 



(3) Tube in about 10.0 cc. lots. 

 Sterilization : Steam for 15 minutes on each 



of 3 successive days. 



Use : To study effect of density on growth of 

 Saccharomyces Zopfii. Cells are smaller 

 in solutions of greatest density. Yeast 

 grown in thick syrups seem to retain 

 their fermentation ability when subjected 

 to high temperatures, more than yeast 

 grown in a medium of lesser density. 



Reference: Owen (1913 p. 473). 



206. Bokorny's Sucrose Ammonium Sul- 

 phate Solution (acid) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Cane Sugar 50.0 g. 



3. KH2PO4 1.0 g. 



4. MgS04 1.0 g. 



5. (NH4)2S04 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Growth of yeast. 

 Reference: Bokorny (1902 p. 56), (1917 p. 

 369). 



207. Mortensen's Sucrose Ammonium 

 Sulphate Solution 



Constituents : 



1. Water 100.0 cc, 



2. Cane sugar (10.0%) 10.0 g. 



3. (NH4)2S04 (0.5%) 0.5 g. 



4. Cobalt salt 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add cobalt salts in varying amounts 

 from 4 to 1/32%. 



Sterilization: Not specified. 

 Use: To study the effect of cobalt salts on 

 the growth of Aspergillus niger. The 



