CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



67 



author found that the toxic properties of 

 cobalt are different in different media. 

 Reference: Mortensen (1909 p. 532). 



208. Woltje's Sucrose Ammonium Sulphate 



Solution (Zikes) 



Constituents: 



1. Water 100.0 cc. 



2. (NH4)2S04 1.0 g. 



3. MgS04 0.25 g. 



4. Saccharose 7.5 g. 



5. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not given. 



Use: To study perithecium formation by 



Aspergillus oryzae. 

 Reference: Zikes (1922 p. 340). 



209. Bokomy's Ethyl Alcohol Ammonium 



Sulphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Ethyl alcohol (0.2%) 20.0 g. 



3. (NH4)2S04 (0.02%) 0.2 g. 



4. K2HPO4 or KH2PO4 (0.05%) 0.5 g. 



5. MgS04 0.2 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not given. 



Use: Growth of fungi, Schizomycetes, 



molds and yeast. 

 Reference: Bokorny (1917 p. 195). 



210. Bokomy's Glycerol Ammonium Sul- 



phate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerin 5.0 g. 



3. KH,P04 0.1 g. 



4. Bittersalts MgS04 0.1 g. 



5. (NH4)2S04 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not given. 



Use: Cultivation of yeast. 

 Reference: Bokorny (1911 p. 182). 



211. Behrens' Basal Malic Acid Ammonium 



Phosphate Solution 



Constituents: 



1. W^ater 1000.0 cc. 



2. (NH4)2S04 20.9 g. 



3. Malic acid 90.0 g. 



4. MgS04 5.0 g. 



5. Potassium phosphate 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 10.0 cc. lots. 



(3) Add 1.0 g. of one of the added nu- 

 trients to each tube. 



Sterilization: Usual method. (Method not 



given.) 

 Use: Cultivation of Penicillium glaucum 



and Botrytis vulgaris. Growth was more 



lu.xuriant in medium containing pectin 



than in arabinose medium. 

 Added nutrients: The author added 1.0 g. 



of arabinose or calcium pectate (from 



flax) to each 10.0 cc. lot. 

 Variants: The author made no additions to 



the basic solution. 

 Reference: Behrens (1898 p. 552). 



212. Dombrowski's Glucose Ammonium 

 Sulphate Solution. 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.5 g. 



4. Dextrose 100.0 g. 



5. Lactic acid 



6. (NH4)2S04 0.5 to 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Acidify slightly with lactic acid 



(3) Add 0.05, 0.1, 1.0, 2.0 or 5.0 g. of 

 (NH4)2S04 to 100.0 cc. of (2). 



Sterilization: Not specified. 



Use: To study nitrogen requirements for 

 milk yeasts, Saccharoinyces Inciis and 

 Zygosaccharomyces lactis. Peptone was 

 found to be the best nitrogen source. 



Variants: The author substituted 20.0 to 

 50.0 g. of asparagin or 2.0 to 50.0 g. of 

 Witte's peptone for the (NH4)2S04. 



Reference: Dombrowski (1910 p. 393). 



213. Bokorny's Tartrate Ammonium 

 Sulphate Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Cane sugar (10.0%) 100.0 g 



3. Ammonium tartrate (0.5%) 5.0 g. 



4. K2HPO4 (0.035%) 0.35 g. 



5. MgS04 (0.006%) 0.06 g. 



6. CaCla (0.0015%o) 0.015 



7. (NH4)2S04 (0.0061%) 0.061g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 



