CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Not given. 



Use: Cultivation of yeast. The culture 



may or may not be treated with a current 



of air. 

 Reference: Bokorny (1917 p. 368). 



214. Raulin's Solution (Lode) 



Constituents : 



1. Water 1500.0 cc 



2. Sugar candy 70.0 g 



3. Tartaric acid 4.0 g 



4. Ammonium phosphate 0.6 g 



5. CaCOs 0.6 g 



6. MgCOs 0.4 g 



7. (NH4)2S04 0.25 g 



8. FeS04 0.07 g 



9. ZnS04 0.07 g 



10. Calcium silicate 0.07 g 



11. Ammonium acetate 4.0 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8, 



9, 10 and 11 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of molds, Aspergillus. 

 Reference: Lode (1902 p. 125). 



215. Proskauer and Beck's Basal Citrate 



Ammonium Sulphate Solution 



(Mendel) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 3.0 g. 



3. Citric acid 2.0 g. 



4. MgS04 2.0 g. 



5. Potassium biphosphate 5.0 g. 



6. Glycerin 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjustment of reaction not specified. 



(3) Add 1.0% of one of the carbon sources 

 listed under added nutrients. 



Sterilization: Sterilize for 30 minutes on 

 each of two successive days. On the 

 third day add 1.0% of glucose and steri- 

 lize for 15 minutes. (Method of steriliza- 

 tion not specified.) 



Use: Cultivation of Bacterium coli and 

 Bacterium Fitzianus. 



Added nutrients: The author used 1.0% of 

 the following carbohydrates as carbon 

 sources: 



glucose maltose 



lactose sucrose 



Reference: Mendel (1911 p. 296). 



216. Pasteur's Glucose Solution 

 (Simanowsky) 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 5.0 g. 



3. (NH4)H2P04 5.0 g. 



4. Glucose 80.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization : Not given. 



Use: Cultivation of yeast. 



Variants: The author substituted 80.0 g. of 



sucrose for glucose. 

 Reference: Simanowsky (1886 p. 22). 



217. Omeliansky's Glucose Ammonium 

 Phosphate Solution 



Constituents 



1. Water 1000.0 cc. 



2. Glucose 100.0 g. 



3. Ammonium phosphate 2.0 g. 



4. KCl 0.1 g. 



5. MgS04 0.1 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Kind of phosphate salt not specified. 



(3) Adjustment of reaction not specified. 



(4) Place solution in thin layer in Wino- 

 gradsky flask. 



Sterilization: Method not given. 



Use: Cultivation of aroma producing yeast 



and the preservation of stock cultures. 



Gives table wine aroma. Cane sugar 



cannot replace glucose. 

 Reference: Omeliansky (1923 p. 418). 



218. Henneberg's Sucrose Ammonium Acid 

 Phosphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. (NH4)2HP04 (0.2%) 2.0 g. 



3. KH2PO4 (0.2%) 2.0 g. 



4. MgS04 (0.1%) 1.0 g. 



5. Sucrose (15.0%) 150.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



Sterilization: Not specified. 



Use : Cultivation of yeast. 



Variants: The author suggested the addi- 

 tion of one of the following: 



Gypsum (2.0%) 20.0 g. 



Potassium phosphate (2.0%) 20.0 g. 



CaCl2 (0.6%) 6.0 g. 



Potassium lactate (2.0%) 20.0 g. 



