CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



69 



K2CO3 (2.0%) 20.0 g. 



Soda (0.6%) 6.0 g. 



K2SO4 (0.6%) 6.0 g. 



Reference: Henneberg (1909 p. 105). 



malic acid 

 citric acid 

 succinic acid 

 Reference: Schukow (1896 p. 607). 



219. Kossowicz's Sucrose Ammonium Phos- 

 phate Solution (Will) 



Constituents: 



1. Water 1000.0 cc. 



2. Saccharose (5.0%) 50.0 g. 



3. KCl (0.4%) 4.0 g. 



4. (NH4)2HP04 (0.4%) 4.0 g. 



5. MgS04 (0.4%) 4.0 g. 



6. CaHP04 (0.04%) 0.4 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5, and 6 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of non-spore forming 



Saccharormjces. 

 Reference: Will (1908 p. 387). 



220. Schukow's Glucose Ammonium 

 Phosphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Ammonium phos- 

 phate 5.0 g. 



3. Potassium phos- 

 phate 10 g. 



4. MgS04 0.5 g. 



5. Glucose 10.0 g. 



6. Organic acid 90.0 g. to 100.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 9 to 10.0% of an organic acid to 



(1). 



(3) Adjustment of reaction not specified. 



(4) Distribute into fermentation flasks 

 (sealed with H2SO4). 



(5) After sterilization and inoculation 

 plug the flasks with a cork and sear 

 with paraffin. 



Sterilization: Sterilize in the steamer 

 (method not given). 



Use : To study utilization of acids by yeast. 

 After 75 days 0.11 g. tartaric, 0.193 g. 

 malic, 0.34 g. citric and 0.09 g. succinic 

 acid were used. Acid determined by 

 titration with N/10 NaOH using litmus 

 as an indicator. 



Variants: The author used one of the fol- 

 lowing organic acids: 



tartaric acid 



221. Rahn's Fat-Ammonium Phosphate 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. (NH4)3P04 5.0 g. 



4. MgS04 1.0 g. 



5. CaCl2 1.0 g. 



6. FeCls trace 



7. HCl trace 



8. Fat 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Melt fat and pour in an Erlenmeyer 

 flask and incline the flask in such a 

 position so that when it solidifies only 

 a portion of the fat will be immersed 

 in the solution. 



(3) Neutralize (1) with 10.0% NaOH 

 using litmus as an indicator. Do not 

 filter. 



(4) Add (3) to the flasks containing fat, 

 solidified on the wall of the flask. 

 Do not add enough (3) to immerse 

 all the fat. 



Sterilization: Not specified. 

 Use: To study fat decomposition by 

 Penicillium glaucum. Fat was decom- 



Reference: Rahn (1905 p. 423). 



222. Bokomy's Ammonium Tartrate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium tartrate . . 2.0 g. 



3. KH2PO4 1.0 g. 



4. MgS04 0.5 g. 



5. Iron chloride trace 



6. CaS04 0.0 or 1.0 g. 



Preparation: Dissolve 2, 3, 4, 5 and 6 in 



water that has been distilled in quartz 



containers. 

 Sterilization: Not specified. 

 Use: To study effect of metallic salts on 



j'east. No growth took place in the 



solution lacking calcium. 

 Reference: Bokorny (1912 p. 135). 



