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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



224. Pasteur's Sucrose Ammonium Solution 



(Pringsheim) 



Constituents : 



1. Water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. Ammonium tartrate 10.0 g. 



4. KH2PO4 5.0 g. 



5. Ca3(P04)2 1.0 g. 



6. Mg3(P04)2 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To study bios requirements of yeast. 



Fermentation took place after one day. 

 Reference: Pringsheim (1906 p. 114). 



225. Bokomy's Sucrose Ammonium Tartrate 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Saccharose (Kahlbaum 



10.0%) 100.0 g. 



3. NaK2P04 (0.1%) 1.0 g. 



4. Ammonium tartrate (0.25%) 2.5 g. 



5. MgS04 (0.05%) 0.5 g. 



6. KCl 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 0.0%, 0.025%, 0.05%, 0.1%, 

 0.25%, 0.5% or 1.0%, KCl to 1. 



Sterilization: Not specified. 



Use: To study effect of metalic salts on 



yeast. Potassium reported necessary for 



the growth of the yeast. 

 Reference: Bokorny (1912 p. 123). 



226. Pringsheim's Sucrose Ammonium 

 Tartrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 2.0 g. 



3. MgS04 0.4 g. 



4. CaCla 0.2 g. 



5. Ammonium tartrate 10.0 g. 



6. Sucrose 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To study bios requirements of yeast. 



The author reported fermentation after 



one day in this medium. 

 Reference: Pringsheim (1906 p. 114). 



227. Hulton-Frankel, Barber and Pyle's 



Acetic Acid and Ammonia Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. H3PO4 (12.79 g) M soln 129.5 cc. 



3. CH3COOH (1.13 g) M soln. . 18.8 cc. 



4. NH4OH (0.62 g) M soln 17.8 cc. 



5. 0.01% CaClo (0.001 g) 10.0 cc. 



6. 0.01% FeCU (0.001 g) 10.0 cc. 



7. 0.01% MgS04 (0.001 g) . . . . 10.0 cc. 



8. NaOH (4.0 g) M soln 100.0 cc. 



9. KOH (5.6 g) M soln 100.0 cc. 



Preparation : 



(1) Dilute solutions 23, and 4 to 500.0 cc. 

 with 1. 



(2) Add to (1), 5, 6 and 7 and equal vol- 

 umes of M solution of 8 and 9 to give 

 pH of 7.0. 



(3) Solution is water clear. 



(4) Tube into sterile tubes. 



(5) pH of medium may be adjusted by 

 varying amounts of alkali added. 

 Medium may be prepared using molar 

 solutions or by weight. 



Sterilization : Steam on each of 3 successive 

 days for 15 minutes. 



Use: General culture medium. 



Variants : Hulton-Frankel and Barber used 

 the same solution as a basic medium with 

 litmus or Andrade's indicator to which 

 they added 10.0 g. of one of the following: 



(a) sucrose (c) lactose 



(b) glucose (d) mannitol 

 References: Hulton-Frankel-Barber and 



Pyle (1917 p. 17), Hulton-Frankel and 

 Barber (1917 p. 17), Harvey (1921-22 

 p. 104). 



228. Wherry's Levulose Ammonium 

 Chloride Solution. 



Constituents : 



1. Redistilled water 1000.0 cc. 



2. NH4CI 2.0 g. 



3. Levulose 2.0 g. 



4. Calcium phosphate 2.0 g. 



(primary, secondary or tertiary) 

 Preparation : 



(1) Dissolve 2, 3 and one of 4 in 1. 



(2) Adjustment of reaction not given. 

 Sterilization: Autoclave (time not spec- 

 ified). 



Use : To study acid proofness of B. tubercu- 

 losis. Primary salt — no growth; sec- 



