CULTURE MEDIA FOR CULTIVATION OF MICRO ORGANSIMS 



71 



ondary salt — growth good — non-acid 

 proof rods, some "free spores." Ter- 

 tiary salt — growth good — smaller non- 

 acid proof rods, many "free spores." 

 Variants: The author gave the following 

 variants: 



(a) Used the same solution but substi- 

 tuted primary, secondary, tertiary 

 meta or pyro sodium phosphate for 

 calcium phosphate. These solutions 

 gave the following results: 



Primary salt growth good at 48 hours — 



part are strongly acid proof, some 



"spores." 

 Secondary salt growth good at 48 hours 



— part are strongly acid proof, some 



"spores." 

 Tertiary salt growth good. Non-acid 



proof. 

 Meta no growth. 

 Pyrophosphate growth good. Rods 



are chiefly short and non-acid proof. 



Some are larger and longer and are 



strongly acid proof. 



(b) Substituted 2.0 g. K2HPO4 for cal- 

 cium phosphate. Bacilli non-acid 

 proof. 



(c) Substituted 2.0 g. KH2PO4 for cal- 

 cium phosphate. Acid proof rods 

 were often club shaped. 



Reference: Wherry (1913 p. 150). 



229. Schardinger's Sucrose Ammonium 



Chloride Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Sucrose 50.0 to 80.0 g. 



3. NH4CI 5.0 g. 



4. KH2PO4 1.0 g. 



5. MgS04 0.5 g. 



6. CaCOs 10.0 to 15.0 g. 



Preparation: (1) Dissolve 2, one of 3, 4, 5 



and 6 in 1. 



Sterilization: Not specified. 



Use: Study of slime production. Fer- 

 mentation starts after 24 hours. The 

 slime forms at the bottom of the flask. 



Variants: The author suggested the use of 

 (NH4)2S04 instead of NH4CI. 



Reference: Schardinger (1902 p. 177). 



230. Klecki's Lactose Ammonium Phosphate 



Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Lactose 20.0 g. 



3. CaCOs 3.0 



4. NH4CI 2.0 



5. Potassium phosphate 0.0.58 



6. Ammonium phosphate . . . 0.1053 g, 



7. MgS04 0.055 g, 



8. (NH4)2S04 0.023 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in Pasteur fermentation 

 tubes. 



Sterilization : Method not given. 



Use: Cultivation of Bacillus saccharo- 

 butyricus. Fermentation takes place after 

 about 60 hours after inoculation. 



Reference: Klecki (1896 p. 255). 



231. Krainsky's Starch Ammonium Chloride 

 Solution. 



Constituents : 



1. Water 1000.0 cc. 



2. Starch (1.0%) 10.0 g. 



3. NH4CI (0.05%) 0.5 g. 



4. K2HPO4 (0.05%) 0.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Isolation and enrichment of Actino- 

 myces. After 2 days because of bacterial 

 increase, a weak coloration and a slight 

 H2S odor appears. The actinomycetes 

 colonies swim on the surface. Inoculate 

 these colonies in a new medium of the 

 same composition and a heavy scum is 

 formed. 



Variants: The author substituted 2 to 3.0% 

 cellulose for 1.0% starch. 



Reference: Krainsky (1914 p. 657). 



232. Vierling's Cellulose Ammonium 

 Chloride Solution 



Constituents : 



1. Water 1000.0 cc. 



2. CaC03 (0.1%) 1.0 g. 



3. (NH4) CI (0.1%) 1.0 g. 



4. Filter paper. 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Place strips of filter paper in flasks. 



(3) Pour (1) into each flask containing 

 filter paper. 



(4) Seal the flasks with paraffin after 

 inoculation. 



Sterilization: Not specified. 



Use: Decomposition of cellulose by Mycch 



