72 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



bacteria. Growth occurs. Filter paper 

 shows no signs of being attacked. 

 Reference: Vierling (1920 p. 206). 



233. Proskauer and Beck's Basal Glycerol 



Ammonium Chloride Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glycerol (1.5%) 15.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. MgS04 (0.25%) 2.5 g. 



5. NH4CI (0.2%) 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the listed carbon sources. 

 Sterilization not specified. 



Use : To study requirements for growth for 



the tubercle bacilli. 

 Added nutrients: The author added the 

 following carbohydrates and alcohols 

 as carbon sources: 

 maltose 1.0% mannitol 0.6% 



lactose 1.0% dulcitol 0.6%, 



sucrose 1.0% isodulcitol 0.6% 



rafhnose 1.0% 

 Reference: Proskauer and Beck (1894 p. 

 146). 



234. Higgins' Glycerol Ammonium Chloride 



Solution 



Constituents : 



1. Water 100.0 cc. 



2. Glycerin 5.0 g. 



3. NH4CI 0.8 g. 



4. Potassium phosphate 0.15 g. 



5. Potassium sulphate or 0.1 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 and 5 in 1. 



(2) Neutralize to phenolphthalein. 

 Sterilization: Method not given. 

 Use: Cultivation of cholera organisms. 

 Reference: Higgins (1898 p. 666). 



235. Revis' Glycerol Ammonium Chloride 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NH4CI 10.0 g. 



3. Glycerol 10.0 g. 



4. Potassium phosphate 10 g. 



5. MgS04 0.2 g. 



6. CaCl2 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: To study stability of physiological 

 properties of colon and coli form organ- 

 isms. No distinctive changes were pro- 

 duced in this non-albuminous medium, 

 but the vigor of fermentation varied 

 somewhat. The organisms show a greater 

 tendency to die off in this medium than 

 in ordinary media. 



Reference: Revis (1910 p. 173). 



236. Proskauer and Beck's Mannitol Ammo- 

 nium Chloride Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Mannitol (0.6%,) 6.0 g. 



3. NH4CI (0.2%) 2.0 g. 



4. KH2PO4 (0.5%) 5.0 g. 



5. MgS04 (0.25%) 2.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use : To study growth requirements of the 

 tubercle bacillus. The authors reported 

 no growth unless glycerol was present. 



Reference: Proskauer and Beck (1894 p. 

 147). 



237. van Delden's Lactate Ammonium 



Chloride Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. NaCl 30.0 g. 



3. K2HPO4 0.05 g. 



4. NH4CI 1.0 g. 



5. Sodium lactate 10.0 g. 



6. MgS04-7H20. . . (amount not specified) 

 Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To study sulphate reduction by M. 



aestuarii. 

 Variants: The author used 0.25 g. K2HPO4, 



0.25 g. NH4CI, specified the use 2.0 g. 



MgS04-7H20 and omitted the NaCl. 

 Reference: van Delden (1903-4 pp. 117- 



118). 



238. Sohngen's Basal Ammonium Chloride 



Salt Solution. 

 Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 0.2 g. 



3. NH4CI 0.2 g. 



4. Seignettes salt (sodium po- 

 tassium tartrate) 0.2 g. 



