CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



73 



Preparation : 



(1) Dissolve 2, 3, and 4 in 1000.0 cc. of 

 water. 



(2) Add one of the materials listed under 

 added nutrients. 



(3) Adjustment of reaction not given. 



(4) Sterilize the solution and colloids 

 separately. 



(5) Incubate 24 hours to test sterility. 

 Sterilization: Method not specified. 



Use: To study influence of colloids on bac- 

 terial process of B. fluorescens lique- 

 faciens. After two days cultures in (2) 

 (a), (c), (e), (h) are turbid white, (d), 

 (f), (g) are clear. Growth obtained in 

 all cases however. 



Added nutrients: The author added one of 

 the following combinations: 



(a) 5.0 g. distilled water 



(b) 5.0 g. distilled water + 0.5 g. blood 

 charcoal 



(c) 5.0 g. distilled water + 0.5 g. blood 

 charcoal + 0.2 g. CaCOs 



(d) 5.0 g. distilled water + 0.5 g. finely 

 divided peat 



(e) 5.0 g. distilled water + 0.5 g. finely 

 divided peat + 0.2 g. CaCOa 



(f) 5.0 g. distilled water + 0.5 g. finely 

 cut filter paper 



(g) 5.0 cc. of a 2.0% colloidal silicum 

 dioxide solution 



(h) 5.0 cc. of a 1.0% colloidal iron oxide 

 solution 

 Reference: Sohngen (1913 p. 625). 



239. Wherry's Ammonium Acetate Solution. 



Constituents : 



1. Redistilled water 1000.0 cc. 



2. NasCOsor NaCl 1.0 g. 



3. KCl 1.0 g. 



4. CaCla 1.0 g. 



5. MgS04 1.0 g. 



6. Ammonium acetate. . . 2.0 g. 



7. NH4CI 2.0 g. 



8. KH2PO4 0.0 or 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Adjustment of reaction not given. 

 Sterilization: Sterilize in autoclave, time 



not given. 



Use : To study acid proofness of B. tubercu- 

 losis. Medium unfavorable. 



Reference: Wherry (1913 p. 148). 



240. Sohngen's Hydrocarbon Ammonium 

 Chloride Solution. 



Constituents : 



1. Water lOCO.O cc. 



2. K0HPO4 0.5 g. 



3. NH4CI 0.5 g. 



4. CaCOs trace 



5. Paraffin (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, and 4 in 1. 



(2) Heat (1) slightly and add 1.0% melted 

 paraffin. Shake thoroughly and then 

 cool quickly. Or add several drops 

 of petroleum. 



(3) Distribute in 100.0 cc. lots in 450.0 cc. 

 Erlenmeyer flasks. 



(4) Inoculate with garden soil. 

 Sterilization: Not specified. 



Use: Cultivation of paraffin oxidizers. Soil 



forms. B. fluorescens liquefaciens, B. 



punctatum, Microcossus paraffmae, B. 



lipolyticum. 

 Variants : Variants used to study the effect 



of a colloid upon oxidation. 



(a) The author substituted several drops 

 of petroleum for paraffin. 



(b) The author substituted 20.0 cc. of 

 petroleum for the paraffin and added 

 2.0 g. of colloidal silicon dioxide or 

 colloidal iron oxide to the solution. 

 He found that the amount of CO2 

 formed was increased by the presence 

 of a colloid, when using Mycobac- 

 terium album, Mycobacterium rubrum, 

 Micrococcus paraffinae. 



Reference: Sohngen (1913 p. 597), (1913 

 p. 644. 



242. Proskauer and Beck's Glucose Ammo- 

 nium Chloride Solution. 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose (1.0%) 10.0 g. 



3. Citric acid (0.075%) 0.75 g. 



4. NH4CI (0.2%) 2.0 g. 



5. Glycerol (1.5%) 15.0 g. 



6. KH2PO4 (0.5%) 5.0 g. 



7. MgS04 (0.25%) 2.5 g. 



8. K2SO4 (0.25%) 2.5 g. 



9. NaCl (0.12%) 1.2 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8 



and 9 in 1. 

 Sterilization: Not specified. 



