74 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: To study growth requirements of 



tubercle bacilli. 

 Variants: The author omitted the citric 



acid. 

 Reference: Proskauer and Beck (1894 p. 



142). 



243. Conn's Glycerol Ammonium Chloride 



Solution. 



Constituents : 



1. Water 1000.0 cc. 



2. Glycerol 10.0 cc. 



3. Malic acid 4.0 g. 



4. NH4CI 0.5 g. 



5. K2HPO4 0.5 g. 



6. CaCl2 2.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjust the reaction to about neutral 

 (pH = 7.0) by the addition of NaOH 

 (about 10.0 cc. of normal solution). 



Sterilization: Not specified. 

 Use: Cultivation of Actinomycetes. 

 Variants: The author omitted the CaCOs 

 and used citric acid instead of malic acid. 

 Reference: Conn (1921 p. 7). 



244. Proskauer and Beck's Mannitol Ammo- 



nium Chloride Solution. 



Constituents : 



1. Water 1000.0 cc. 



2. Mannitol (0.6%) 6.0 g. 



3. Glycerol (1.5%) 15.0 g. 



4. NH4CI (0.2%) 2.0 g. 



5. KH2PO4 (0.5%) 5.0 g. 



6. MgS04 (0.25%) 2.5 g. 



7. Magnesium citrate (0.25%). 2.5 g. 



8. Citric acid (0.075%) 0.75 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 



and 8 in 1. 

 Sterilization: Not specified. 

 Use : To study growth requirements of the 



tubercle bacillus. Authors reported no 



growth unless glycerol was present. 

 Reference: Proskauer and Beck (1894 p. 



147). 



244a. Mayer's Ammonium Nitrate Solution 

 (Tanner) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. MgS04-7H20 10.0 g. 



3. NH4NO3 15.0 g. 



4. Ca3(P04)2 0.01 g. 



5. KH2PO4 10.0 g. 



6. Sugar (kind not specified). 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Cool and add sugar (Kind and amount 

 not specified). 



Sterilization: Not specified. 



Use: General culture medium. 



Variants: Add 3.0% NaCl if the medium is 

 to be used for luminous bacteria, and an 

 excess of CaCOs if acid forming bacteria 

 are to be grown. 



Reference: Tanner (1919 p. 62). 



245. Capaldi and Proskauer's Mannitol 



Ammonium Nitrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NH4NO3 (0.2%) 2.0 g. 



3. Mannite (0.2%) 2.0 g. 



4. NaCl (0.02%) 0.2 g. 



5. KH2PO4 (0.02%) 0.2 g. 



6. MgS04 (0.01%) 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Acid production by B. coli. Authors 



found that B. coli produced acid in this 



medium. 

 Reference: Capaldi and Proskauer (1896 



p. 456). 



246. Proskauer and Beck's Glycerol Ammo- 



nium Nitrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glucose (1.0%) 10.0 g, 



3. Glycerol (1.5%) 15.0 g. 



4. KH2PO4 (0.5%) 5.0 g. 



5. MgS04 (0.25%) 2.5 g. 



6. NH4NO3 (0.2%) 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use : To study food requirements of tuber- 

 cle bacilli. 



Reference: Proskauer and Beck (1894 p. 

 142). 



247. Heraeus' Glucose Ammonium Car- 

 bonate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 0.05 g. 



