CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(O 



3. MgS04 0.01 g. 



4. CaCU 0.05 g. 



5. Dextrose 1.0 g. 



6. (NH4)2COo 1.0 g. 



Preparation : 



(1) Dissolve 1.0 g. potassium phosphates 

 0.2 g. MgS04 and 0.1 g. CaCh in 

 1000.0 cc. distilled water 



(2) Steam for 1 hour. 



(3) Allow to stand. 



(4) Filter and boil again. 



(5) Prepare solutions of dextrose and 

 (NH4)2C03, the ratio of 1:15. 



(6) Mix 10.0 cc. (4), 1.0 cc. of the dextrose 

 solution and 1.0 cc. of (NH4)2C02 

 solution. 



(7) Dilute to 200.0 cc. with distilled 

 water. A liter of this solution con- 

 tains: 



0.05 g. potassium phosphate 

 0.01 g. MgSO 

 0.05 g. CaCl2 

 1.0 g. dextrose 

 1.0 g. NH4CO3 



(8) Distribute in 50.0 cc. flasks that have 

 previously been plugged and sterilized 

 in the sterilizer. 



Sterilization : Heat the flasks in the steamer 



for 30 minutes. 

 Use: General culture medium. 

 Reference: Heraeus (1913 p. 224). 



248. Lowenstein's Glycerol Ammonium 

 Carbonate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. (NH4)2C04 (Commercial) ... 3.5 g. 



3. Potassium phosphate 



(primary) 1-5 g. 



4. MgS04 2.5 g. 



5. Glycerin 15.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 

 Reference: Lowenstein (1913p. 592). Har- 

 vey (1921-22 p. 103). 



249. Proskauer and Beck's Basal Glycerol 

 Ammonium Carbonate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glycerol (1.5%) 15.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. MgS04 (0.25%) 2.5 g. 



5. Magnesium Citrate (0.25%). 2.5 g. 



6. (NH4)2C03 (0.3%) 3.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To study constituents essential for 



growth of the tubercle bacilli. 

 Variants : 



(a) The authors used 0.2% (NH4)2C03 

 instead of 0.3% and substituted 0.4% 

 ammonium citrate for 0.25% magne- 

 sium citrate. 



(b) Smith gave the following solution: 



1. Distilled water 1000.0 g. 



2. (NH4)2C03 3.5 g. 



3. KH2PO4 1.5 g. 



4. MgS04-7H20 2.5 g. 



5. Glycerol 15.0 g. 



(c) Tanner gave the same solution as 

 Smith but used K3PO4 instead of 

 KH2PO4. 



References: Proskauer and Beck (1894 p. 

 148), Smith (1905 p. 198), Kolle and 

 Wasserman (1912 p. 394), Tanner (1919 

 p. 68), Klimmer (1923 p. 112). 



250. Beijerinck's Glucose Ammonium Sul- 



phate Solution 



Constituents: 



1. Water 100.0 cc. 



2. KH2PO4 0.05 g. 



3. (NH4)2S04 0.05 g. 



4. Glucose 3.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Streptothrix chrom- 



ogem. 

 Reference: Beijerinck (1900 p. 10). 



251. Doryland's Glucose Ammonium Sul- 



phate Solution 



Constituents: 



1. Distilled water 500.0 cc. 



2. HCl dilute 



3. MgS04 0.5 g. 



4. CaO 0.01 g. 



5. Fe2(S04)3 0.01 g. 



6. MnS04 0.01 g. 



7. (NH4)2S04 1.00 g. 



8. H2SO4 



9. H3PO4 



10. Glucose 10.0 g. 



11. N/ 0.2578 NaOH 



12. N/ 0.6205 KOH 



