CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



77 



(2) Add 1.0 g. of one of 7 to (1). 



(3) Adjustment of reaction not given. 

 Sterilization: In the autoclave. 



Use: To study the cycle of the phosphate 

 ion in the soil using Bac. proteus and 

 vulgaris, Bac. mesentericus vulgatus. 

 PO4 ions were probably formed. 



Reference: Stoklasa (1911 p. 479). 



255. Percival's Glucose Ammonium Sul- 



phate Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 2.0 g. 



3. K2HPO4 1-0 g. 



4. NaCl 2.0 g. 



5. MgS04 0.5 g. 



6. FeS04 0.4 g. 



7. MgCOs 10.0 g. 



8. De.xtrose 0.01, 0.03, 1.0 or (3.0%) 0.1, 

 0.3, 3.0, 10.0 or 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute in 50.0 cc. into conical 

 flasks. 



(3) Add 0.5 g. of basic MgCOs to each 

 flask. 



(4) To each flask add 0.01, 0.03, 0.3, 1.0 

 or 3.0% glucose. 



Sterilization: Not specified. 



Use: To study nitrification by bacteria 



from the soil. 

 Reference: Percival (1920 p. 144). 



256. Lipman, Waksman and Joffe's Glucose 



Sulphur Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)3S04 2.0 g 



3. K2HPO4 10 g 



4. MgS04 0.5 g 



5. KCl 0.5 g 



6. FeS04 0.01 g 



7. Glucose 10.0 g 



8. Sulphur 10.0 g 



9. Ca3(P04)2 10.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 



(2) Adjust to pH between 6.0 and 6.2. 

 Sterilization: Sterilize in the steamer the 



fractional method. 

 Use: Cultivation of organisms capable of 



o.xidizing sulphur. 

 Variants: The authors used only 1.0 g. 



glucose instead of 10.0 g. 



Reference: Lipman, Waksman and Joffe 

 (1921 p. 475) taken from (1922 p. 108). 



257. Bokorny's Sucrose Ammonium Sul- 



phate Solution (Basic) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. K,HP04 5.0 g. 



4. MgS04 10 g. 



5. (XH4)2S04 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: To study yeast growth. 



Variants : Zikes used the modification given 

 below to study volutin formation by wine 

 yeast, Saccharomyces Frohberg, Sac- 

 charomyces anamensis, Saccharomyces 

 ilicis, Saccharomyces, Will (Bajonus) 

 Pichia membranaefaciens, Cholara myco- 

 derma, Monilia Candida, Oidium laclis, 

 Endomyces Magnusii. 



1. Distilled water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. K2HPO4 10 g. 



4. MgS04 0.3 g. 



5. (NH4)2S04 2.5 g. 



(pH = 5.3718, CH = 4.25 X lO--^). 



Reference: Bokorny (1911 p. 183), Zikes 

 (1922 p. 29). 



258. Wellman's Starch Ammonium Sulphate 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. (NH4)2S04 2.0 g. 



3. KNO3 10 g. 



4. K3PO4 2.0 g. 



5. NaCl 2.0 g. 



6. Starch 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add starch to (1). (Amount not 

 given.) 



Sterilization: Not specified. 



Use: Cultivation of Glycobacter proteoly- 



ticus. 

 Reference: Wellman (1912 p. 616). 



259. Heller's Starch Ammonium Sulphate 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Sodium phosphate 0.5 g. 



