78 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Ammonium sulphate 0.5 g. 



4. Soluble starch 10.0 g. 



5. Calcium carbonate 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjustment of reaction not specified. 

 Sterilization: Not specified. 



Use: Enrichment of soil anaerobes. 

 Reference: Heller (1921 p. 453). 



260. Heinze's Glycogen Ammonium Sul- 



phate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KH0PO4 2.0 g. 



3. MgS04 0.4 g. 



4. CaCl. 0.2 g. 



5. Glycogen 10.0 



6. (NH4)2S04 0.1 „ 



7. FeCla (dilute solution) ... 20 drops 

 Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 



7 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Aspergillus and other 



molds. 

 Variants : 



(a) The author used 2.5 g. glycogen 

 instead of 10.0 g. 



(b) The author used 1.3 g. (NH4)2S04 

 and 2.5 g. of glycogen instead of 

 0.1 g. (NH4)2S04 and 10.0 g. of glyco- 

 gen. 



(c) The author used 4.0 g. MgS04, 2.5 g. 

 glycogen and omitted the FeCls 

 solution. 



Reference: Heinze (1904 p. 182). 



261. McBeth's Cellulose Ammonium Sul- 



phate Solution (Sanborn) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KiHP04 1.0 g. 



3. MgS04 1.0 g. 



4. NaoCOs 1.0 g. 



5. (NH4)2S04 2.0 g. 



6. Cellulose 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add a suitable source of cellulose. 

 Sterilization: Sterilize in the autoclave at 



15 pounds pressure. 

 Use: Cultivation of Cellculomonas folia. 

 The author used maple leaves and filter 

 paper as sources of cellulose. He re- 



ported that the presence or absence of an 

 "essential food factor represented by 

 vitamine B (?) exerts a stimulating effect 

 upon the growth in physiological effi- 

 ciency of C. folia." 

 Reference: Sanborn (1926 p. 2). 



262. Lohnis' Ammonium Sulphate Cellulose 



Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. (NH4).2S04 1.0 g. 



3. K.2HPO4 1.0 g. 



4. MgS04 0.5 g. 



5. NaCl trace 



6. CaCOs 20.0 g. 



7. Cellulose 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Fill tubes with (1). 



(3) Add about 0.1 g. of filter paper, 

 cotton wool or straw to each tube. 



Sterilization: Not specified. 



Use: Cultivation of cellulose decomposers. 

 Author inoculated with rotten dung. 



Variants: Cunningham tubed the solution 

 in 15.0 to 20.0 cc. quantities and added a 

 small quantity of finely cut filter paper to 

 each tube. Fermentation tubes with a 

 glass rod inside to hold them above the 

 CaCOs were added. The medium was 

 used to grow anaerobes. 



Reference: Lohnis (1913 p. 93), Cunning- 

 ham (1924 p. 142). 



263. Lohnis' Pectin Ammonium Sulphate 



Solution 



Constituents : 



1. Water (tap) 1000.0 cc. 



2. (NH4)2S04 0.5 g. 



3. K2HPO4 0.5 g. 



4. CaCOa 20.0 g. 



5. Pectin 5.0 g. 



Preparation : 



(1) Grate and extract about 500.0 g. of 

 carrot roots with warm water con- 

 taining chloroform. 



(2) Treat the residue for about 30 minutes 

 (without warming) with dilute caus- 

 tic soda 



(3) Wash (2) with water. 



(4) Extract for 30 minutes with dilute 

 HCl. 



(5) Again wash with water. 



