CULTURE MEDIA FOR CULTIVATIOX OF MICROORGANISMS 



79 



(6) Dissolve the pectin by adding dilute 

 ammonia and precipitate it from 

 solution by the addition of CaCU. 



(7) Thoroly wash the pectin precipitate 

 with distilled water. 



(8) Add 0.1 g. of (7) to a series of flasks. 



(9) Dissolve 2, 3 and 4 in 1. 



(10) Add 2.0 cc. of (9) to each flask of (8). 

 Sterilization: Not specified. 

 Use: Enrichment of B. amylobader . 

 Reference: Lohnis (1913 p. 92). 



264. Beijerinck's Glucose Starch Ammo- 



nium Sulphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 30.0 g. 



3. Starch 1.0 g. 



4. KH2PO4 0.5 g. 



5. (NH4)2S04 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Streptothrix chromo- 

 gena. The author found that the medium 

 supported growth very well. 



Reference: Beijerinck (1900 p. 11). 



265. Proskauer and Beck's Mannitol Glyc- 

 erol Ammonium Sulphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Mannitol (0.6%) 6.0 g. 



3. Glycerol (1.5%) 1.5 g. 



4. KH2PO4 (0.5%) 5.0 g. 



5. MgS04 (0.25%) 2.5 g. 



6. (NH4)2S04 (0.2%) 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To determine constituents essential 



for the growth of the tubercle bacillus. 

 Reference: Proskauer and Beck (1894 



p. 147). 



266. Proskauer and Beck's Basal Glycerol 



Ammonium Sulphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol (1.5%) 15.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. Magnesium citrate (0.25%). 2.5 g. 



5. (NH4)2S04 (0.2%) 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the carbohydrates listed 

 to (1). 

 Sterilization: Not specified. 

 Use: To study the constituents essential 



for the growth of the tubercle bacillus. 

 Added nutrients: 



(a) The authors added one of the follow- 

 ing carbohydrates. 



no added carbon 



raffinose 1-0% 



sucrose 1 .0% 



maltose 1-0% 



lactose 1.0% 



glucose 1.0% 



manno.se 1-0% 



levulose 1.0% 



dulcitol 0.6% 



isodulcitol 



(b) Tiffeneau and Marie added 0.6% man- 

 nitol as a carbon source. 



Reference: Proskauer and Beck (1894 

 pp. 142-146), Tiffeneau and Marie (1912 

 p. 48). 



267. Doryland's Acetic Acid Ammonium 

 Sulphate Solution 



Constituents: 



1. Water 500.0 cc. 



2. NaOH, N/0.2578 



3. KOH, N/0.6205 



4. HCl 



5. MgSOi 0.5 g 



6. CaO 0.01 g 



7. Fe2(S04)3 0.01 g 



8. MnS04 0.01 g 



9. (NH4)2S04 1.0 g 



10. H2SO4 



11. H3PO4 



12. CH3COOH 

 Preparation : 



(1) Dilute the HCl so that 1.0 cc. is not 

 quite neutralized by 1.0 cc. of a 

 silicate solution made by dissolving 

 24.0 g. KaSiOs and 8.4 "g. NaoSiOa 

 in 500.0 g. water. Phenolphthalein 

 is used as an indicator. 



(2) Add to the HCl the following salts: 



MgS04 0.5 g 



CaO 0.01 g 



Fe2(S04)3 0.01 g 



MnS04 0.01 g 



(NH4)2S04 1.0 g 



(3) Standardize the resulting HCl solu- 

 tions against the silicate, using 



