80 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



methyl orange as an indicator, so that 

 1.0 cc. is equivalent to 1.0 cc. of the 

 silicate solution. 



(4) Standardize a solution of H2SO4 in 

 the same way as HCl omitting the 

 salts. 



(5) Standardize H3PO4 and CH3COOH 

 in a similar manner as HCl omitting 

 the salts and using phenolphthalein 

 as an indicator. 



(6) Mix the acids in the following pro- 

 portion: 



HCl 153.5 cc. 



CHrCOOH 153.5 cc. 



H2SO4 77.0 cc. 



H3PO4 116.0 cc. 



(7) Mix equal quantities of N/0.6205 

 KOH and N/0.2578 NaOH. 



(8) 1 cc. of (7) should neutralize 1 cc. of 

 (6) using phenolphthalein as indi- 

 cator. 



(9) Acids and bases are drawn into 

 separate burettes and allowed to 

 stand to sterilize. 



(10) Mix equal amounts when ready to use. 

 Sterilization: Not specified. 

 Use: As a general synthetic culture me- 

 dium. 

 Reference: Doryland (1916 p. 146). 



268. Proskauer and Beck's Basal Citrate 

 Ammonium Sulphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 (0.5%) 5.0 g. 



3. Magnesium citrate (0.25%). 2.5 g. 



4. (NH4)2S04 (0.25%) 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 1.0% of one of the listed carbo- 

 hydrates. 



Sterilization: Not specified. 

 Use: To determine the constituents essen- 

 tial for growth of the tubercle bacillus. 

 Added nutrients: The authors added 1.0% 

 of one of the following carbohydrates: 

 levulose raffinose 



mannose dulcitol 0.6% 



maltose isodulcitol 0.6% 



sucrose mannitol 0.6% 



lactose 

 Reference: Proskauer and Beck (1894 

 pp. 144-146). 



269. Peglion's Sucrose Ammonium Sulphate 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Cane sugar 50.0 g. 



3. Cream of tartar 10 g. 



4. MgS04 0.3 g. 



5. (NH4)2S04 0.2 g. 



Preparation : (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of B. oncidii. 

 Reference: Peglion (1899 p. 36). 



270. Higgins' Succinate Ammonium Sul- 

 phate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 2.5 g. 



3. Lactose 2.5 g. 



4. Ammonium succinate 2.5 g. 



5. (NH4)2S04 1.0 g. 



6. KCl 1.5 g. 



7. KNO3 2.5 g. 



8. Ammonium phosphate 2.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 



and 8 in 1. 



Sterilization: Method not specified. 



Use: Cultivation of organisms causing 

 cholera, Picarton cattle disease and other 

 organisms. The author found that this 

 medium gave approximately the same 

 results as ordinary broth. 



Reference: Higgins (1898 p. 668). 



271. Lockemann's Citrate Ammonium Sul- 

 phate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaH2P04-H20 3.0 g. 



3. KH2PO4 4.0 g. 



4. (NH4)2S04 5.0 g. 



5. Magnesium citrate 2.5 g. 



6. Glycerin 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) The reaction should be acid titer 

 1/100 N 3.3. 



(3) Distribute in 50.0 cc. lots in flasks. 

 Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 

 Reference: Lockemann (1919 p. 421). 



