CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



81 



272. Beijerinck's Glucose Ammonium 

 Phosphate Solution 



Constituents; 



1. Water 1000.0 g. 



2. Glucose 20.0 g. 



3. K2HPO4 0.2 g. 



4. (XH4)2HP04 0.2 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: The author found that Azolobocter 

 chrnococcum utilized ammonium salts 

 readily in this medium. 



Reference: Beijerinck (1901 p. 575). 



273. Ayer's Glucose Ammonium Phosphate 



Solution (Caldwell) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Sodium ammonium phos- 

 phate 4.0 g. 



3. NaHzPOi 2.0 g. 



4. Glucose 10.0 g. 



5. Marble 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Prepare a 20% glucose solution in 

 distilled water. 



(3) Mix sterile (1) and sterile (2). 



(4) Tube in sterile tubes containing a 

 little ground marble. 



Sterilization: Method not given. 



Use : To study anticolon bacteriophage in a 



synthetic medium. 

 Reference: Caldwell (1926 p. 123). 



274. Kendall, Day and Walker's Glucose 



Ammonium Phosphate Solution 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. (XH4)2HP04 4.0 g. 



3. NaCl 5.0 g. 



4. Glucose 10.0 g. 



Preparation: 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjustment of reaction not given. 

 Sterilization: Not specified. 



Use: To study the metabolism of the 

 tubercle bacillus. Alizarin, neutral red 

 and phenolphthalein were used to study 

 changes in reaction; Ziehl-Nielsen stain 

 for staining. Kendall, Walker and Day 

 used the solution for the study of lipase 

 production by the tubercle bacillus. Dif- 



ferent esters with clear bacteria free 

 culture broth were incubated for 24 hours; 

 the amount of acid produced (measured 

 in terms of N/50 NaOH), determined 

 lipase production. 

 References: Kendall, Day and Walker 

 (1914 p. 434), Kendall, Walker and Day 

 (1914 p. 455). 



275. Wherry's Levulose Sodium Ammonium 



Phosphate Solution 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. Levulose 2.0 g. 



3. Sodium ammonium phos- 

 phate 2.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 



Sterilization: Sterilize in autoclave (time 

 not given). 



Use: To study acid proof ness of B. tuber- 

 culosis. Author reported good growth, 

 but the organisms stain irregularly and 

 are not acid proof. 



Reference: Wherry (1913 p. 150). 



276. Wolffin's Glucose Ammonium Phos- 



phate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Dextrose 100.0 g. 



3. Potassium phosphate 10 g. 



4. Ammonium phosphate 10.0 g. 



5. MgS04 0.2 g. 



6. CaCOs 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjustment of reaction not specified. 



(3) Distribute in flasks in 250.0 cc. lots. 



(4) Add 1.0 g. CaCOs to each flask. 

 Sterilization: Not specified. 



Use: To study fermentation by Bacillus 



levans. 

 Reference: Wolffin (1894 p. 288). 



277. Stutzer's Sucrose Ammonium Magne- 



sium Phosphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 20.0 g. 



3. Potassium phosphate 1.0 g. 



4. Ammonium magnesium 

 phosphate (dry) 

 (MgNH4P04)." 2.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 



