CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Not specified. 



Use: Cultivation of bacteroids from Pis urn 



sativum. 

 Reference: Stutzer (1900 p. 902). 



278. Henneberg's Sucrose Ammonium 

 Phosphate Solution 



Constituents : 



1. Distilled water 1000.0 g 



2. K0HPO4 (0.5%) 5.0 g 



3. xMgS04 (0.2%) 2.0 g 



4. Cane sugar (5.0%) 50.0 g 



5. Ammonium phosphate (0.3%) 3.0 g 

 Preparation: (1) Dissolve 2, 3, 4 and 5 in 1 

 Sterilization: Not specified. 



Use: Cultivation of yeast. 

 Reference: Henneberg (1907 pp. 40-45), 

 (1909 p. 104). 



279. Botkin's Starch Ammonium Phosphate 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Ammonium phosphate 0.08 g. 



3. Potassium phosphate 0.04 g. 



4. MgS04 0.04 g. 



5. (NH4)2S04 0.02 g. 



6. Starch, potato 30.0 g. 



7. CaC03 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add 7 to (1). 

 Sterilization: Not specified. 



Use: To ascertain fermentation of starch 

 by Bacillus hutyricus. The author found 

 that gas was produced from the starch 

 after 24 hours and that the culture 

 reduced Fehling's solution. 



Reference: Botkin (1872 p. 431). 



280. Waksman and Carey's Ammonium 



Phosphate Cellulose Solution 



Constituents : 



1. Distilled water 



2. (NH4)2HP04 



3. MgS04 



4. FeS04 



5. KCl 



100.0 cc 



5.0 g 



1.0 g 



0.02 g 



1-0 g 



6. Cellulose (filter paper) 4.0 g 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Grind filter paper. I'| 



(3) Add 1.0 g. ground filter paper to 

 flasks containing 25.0 cc. of sterile (1). 



Sterilization: Method not given. 



Use: Cultivation of Spirochaeta cytophaga 

 and other organisms capable of decom- 

 posing cellulose. 



Variant: The authors used the medium 

 diluting each flask containing 25.0 cc. of 

 the medium with 150.0 cc. of water. 



Reference: Waksman and Carey (1926 

 p. 92). 



281. Percival's Cellulose Ammonium Phos- 

 phate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH.PO4 1.0 g. 



3. Ammonium phosphate 1.0 g. 



4. MgS04 0.5 g. 



5. NaCl a small crystal 



6. Filter paper or Blotting 



paper 3.0 g. 



7. CaCOs 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Pour into 250.0 cc. flasks and add 5.0 g. 

 of CaCOs. 



(3) Cut about 3.0 g. of filter or blotting 

 paper into small pieces and add 

 to (2). 



(4) Inoculate with a piece of fresh horse 

 dung or mud from the bottom of a 

 pond. 



(5) Fill the flasks completely and insert 

 a cork thru which passes a bent glass 

 tube, arranged so as to collect any 

 gas given off. 



Sterilization: Not specified. 



Use: Cultivation of B. amylobacter, a 

 cellulose splitting organism. Author re- 

 ported the production of gas after 3 or 

 4 weeks. 



Reference: Percival (1920 p. 231). 



282. Loew's Formaldehyde Ammonium 

 Phosphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 2.0 g. 



3. (NH4)2HP04 1.0 g. 



4. MgS04 0.1 g. 



5. CaClj 0.1 g. 



6. Sodium salt or formaldehyd- 

 sulfurous acid ("Formalde- 

 hyschwefligsaurem Na- 

 trom") 5.0 g. 



