CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



83 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Isolation of a red bacillus from the 



air capable of utilizing formic acid as a 



carbon source. 

 Reference: Loew (1892 p. 462). 



283. Loew's Methyl Alcohol Ammonium 



Phosphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Methyl alcohol 5.0 g. 



3. K2HPO4 0.5 g. 



4. (NH4)2HP04 0.5 g. 



5. MgS04 0.1% 



Preparation : 



(1) Dissolve 3, 4 and 5 in 1. 



(2) After sterilization add 2 to (1). 

 Sterilization: Method not given. 



Use: Isolation of bacillus from the air, 

 producing red pigment and capable of 

 assimilating formic acid. 



Reference: Loew (1892 p. 463). 



284. Omeliansky's Ethyl Alcohol Ammonium 



Phosphate Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1-0 g. 



3; MgS04 0.5 g. 



4. Ammonium phosphate 1-0 g. 



5. NaCl traces 



6. Ethyl alcohol 10.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute into long necked flasks 

 with lateral tubes. 



(3) Add a small amount of chalk to each 

 tube. 



(4) After sterilization cool. 



(5) Add 1.0 part ethyl alcohol to each 

 100.0 parts medium under aseptic 

 conditions. 



Sterilization: Method not specified. 



Use: Fermentation of ethyl alcohol with 



the production of methane. 

 Reference: Omeliansky (1916 p. 58). 



285. Beijerinck's Alcohol Ammonium Phos- 



phate Solution 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Alcohol 30.0 g. 



3. Ammonium phosphate 0.5 g. 



4. KCl 0.1 g. 



Preparation : 



(1) Dissolve 3 and 4 in tap water. 



(2) Distribute in 10.0 cc. lots in Frenden- 

 reich flasks. 



(3) After sterilization add 3.0% by 

 volume of absolute alcohol and suffi- 

 cient acetic acid to each tube to dis- 

 solve the precipitate. 



Sterilization: Method not given. 

 Use: Cultivation of acetic acid bacteria. 

 References: Beijerinck (1898 p. 214), Janke 

 (1916 p. 6). 



286. De Schweinitz's Glycerol Ammonium 

 Phosphate Solution (Gage and Phelps) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. MgS04 (0.02%) 0.2 g. 



3. K2HPO4 (0.1%) 1.0 g. 



4. Ammonium phosphate 



(1.0%) 10.0 g. 



5. Glycerin (4.0%) 40.0 g. 



6. Neutral red 1.0% water 

 solution (1.0%) 10.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 10.0 cc. of a 1.0% water solution 

 of neutral red to (1). 



Sterilization: Not specified. 



Use: To observe neutral red reaction by 



colon types. B. coli changes the color 



from red to yellow. 

 Reference: Gage and Phelps (1902 p. 411). 



287. Lowenstein's Glycerol Ammonium 

 Phosphate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Ammonium phosphate 6.0 g. 



3. Glycerol 40.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Method not given. 



Use: Cultivation of tubercle bacilli. 

 Tubercle bacilli develop very poorly on 

 this medium. Na, K, CI or S is necessary 

 for growth. 



Variants : 



(a) Lockemann specified the use of 

 (NH4)3P04-3H20 and added 0.6 g. 

 MgSOrTHaO. 



(b) Lockemann specified the use of 

 (NH4)3P04-3H20 and added 0.6 g. 



