84 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



MgS04-7H20 and 3.0 g. Na.HPOi- 

 H2O. 

 References: Lowenstein (1913 p. 592), 

 Lockemann (1919 p. 421). 



288. Capaldi and Proskauer Mannitol 

 Ammonium Phosphate Solution 



Constituents: 



1. Water 



2. Ammonium 

 (0.2%) 



3. Mannite (0.2%). 



4. NaCl (0.02%)... 



5. MgS04 (0.01%) . 



phosphate 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 



Sterilization: Not specified. 



Use: To determine acid production by 



B. coll. 

 Reference: Capaldi and Proskauer (1898 



p. 459). 



289. Omeliansky's Mannitol Ammonium 



Phosphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Mannitol 20.0 g 



3. Potassium phosphate 1-0 g 



4. Ammonium phosphate 1.0 g 



5. MgS04 0.5 g 



6. NaCl 0.2 g 



7. Chalk 20.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add 20.0 g. of chalk. 



(3) After sterilization add chalk to each 

 tube. 



Sterilization: Sterilize at 115°C. 



Use: To study fermentation of mannitol 



by Bact.formicicum. 

 Reference: Omeliansky (1903 p. 319). 



290. Loew's Formate Ammonium Phosphate 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH0PO4 2.0 g 



3. (NH4)2HP04 1.0 g 



4. MgS04 0.1 g 



5. CaCla 0.1 g 



6. Sodium formate 5.0 g 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To show the assimilation of formic 



acid by a bacillus producing a red pigment 

 isolated from the air. Pure formic acid 

 gave the same results. 

 Reference: Loew (1892 p. 463). 



291. Stutzer's Citrate Ammonium 

 Phosphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Ammonium phosphate 2.0 g. 



3. Mg(N03)2 2.0 g. 



4. Citric acid 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Bacteroids from Pisum 



sativum. 

 Reference: Stutzer (1906 p. 901). 



292. Koser's Citrate Sodium Ammonium 



Phosphate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Na(NH4)HP04-4H20 (mi- 

 crocosmic salt) 1-5 g. 



3. KH2PO4 1.0 g. 



4. MgS04 0.2 g. 



5. Sodium citrate 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) pH is about 6.7 to 6.9. 



(3) Distribute in 5.0 to 8.0 cc. quantities. 

 Sterilization: In the autoclave. 



Use: Differentiation of colon-aerogenes 

 group. Bad. coli of fecal origin is 

 unable to utilize citric acid as a source of 

 carbon, while the aerogenes-cloacae tj^pes 

 utilize citric acid. 



Variants : 



(a) Potassium citrate may replace the 

 sodium salt. Ammonium citrate is 

 quite unstable. 



(b) A. P. H. A. used from 2.5 to 3.0 g. 

 sodium citrate. 



Reference: Koser (1924 p. 63), American 

 Public Health Assoc. (1925 p. 112). 



293. Koser's Citrate Ammonium Phosphate 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaCl 5.0 g. 



3. AIgS04 0.2 g. 



4. NH4H2PO4 1.0 g. 



