CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



85 



5. K2HPO4 1.0 g. 



6. Sodium citrate 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. (Use 

 2.77 g. sodium citrate 5J H2O.) 



(2) pH is about 6.7 to 6.9. 



(3) Distribute in 5.0 to 8.0 cc. quantities. 

 Sterilization: In the autoclave. 



Use: Differentiation of the colon-aero- 

 genes group. Bact. coli of fecal origin is 

 unable to utilize citric acid as a source 

 of carbon, while the aerogenes-cloacae 

 types utilize citric acid. 



Variants: Potassium citrate may be used 

 instead of sodium citrate. Ammonium 

 citrate is quite unstable. 



Reference: Koser (1924 p. 63). 



294. Tausz and Peter's Paraffin Magnesium 

 Ammonium Phosphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. MgNH4P04 (0.1%) 1.0 g. 



3. CaS04 (0.01%) 0.1 g. 



4. K2HPO4 (0.08%) 0.8 g. 



5. FeCls trace 



6. KI trace 



7. Paraffin (amount not specified). 

 Preparation: (1) Mix 1, 2, 3, 4, 5, 6 and 7. 



Complete solution does not take place. 

 Sterilization : Steam by the Klocker method 



on 2 successive days for 30 minutes each 



day. 

 Use: Cultivation of Bacterium aliphat- 



icum, Bacterium aliphatic um . lique- 



faciens, paraffin bacteria. 

 Reference: Tausz and Peter (1919 p. 507). 



295. Sohngen's Methane Magnesium 

 Ammonium Phosphate Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. MgNH4P04 1.0 g. 



4. CaS04 0.1 g. 



5. Methane 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Flask and inoculate (garden earth, 

 dung water or ditch water may be 

 used for inoculation). 



(3) Fill the flask with a known mixture 

 of oxygen and methane. 



Sterilization: Not specified. 



Use: Isolation and enrichment of Bacillus 

 methanicus (utilizing methane). 



References: Sohngen (1905 p. 514), Lohnis 

 (1913 p. 105). 



296. Laurent's Sucrose Ammonium Phos- 



phate Solution (Pringsheim) 

 Constituents : 



1. Water 1000.0 cc. 



2. Sugar 100.0 g. 



3. Tartaric acid 1.0 g. 



4. KH2PO4 0.75 g. 



5. MgS04 0.1 g. 



6. (xNH4)3P04 5.0 g. 



Preparation: Dissolve 2, 3, 4, 5 and 6 in 1. 

 Sterilization: Not specified. 



Use: To study the bios requirements of 

 yeast. The author reported that fer- 

 mentation occurred after 2 months. 



Reference: Pringsheim (1906 p. 114). 



297. Janke's Alcohol Ammonium Phosphate 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.4 g. 



3. (NH4)2HP04 1.0 g. 



4. MgS04 + 7H2O 0.4 g. 



5. Glycerin 5.0 cc. 



6. Succinic acid 1-0 g. 



7. Alcohol (3.0%) 30.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in distilled 

 water. 



(2) Add distilled water to (1) to make 

 1000.0 cc. 



(3) After sterilization add 3.0% by 

 volume of alcohol. 



Sterilization: Method not given. 



Use: Cultivation of acetic acid bacteria. 



Variants : H3PO4 or acetic acid may be used 

 instead of succinic acid. The acid is used 

 for the purpose of keeping the materials 

 in solution. Succinic acid does not in- 

 hibit growth whereas the other mentioned 

 acids do. 



Reference: Janke (1916 p. 7), (1921 p. 91). 



298. Wherry's Ammonium Acetate Salt 

 Solution 



