86 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



4. CaClo 1.0 g. 



5. MgS04 1.0 g. 



6. KH2PO4 2.0 g. 



7. Ammonium acetate 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Adjustment of reaction not specified. 

 Sterilization: In the autoclave (time not 



given). 



Use: To study acid proof ness of B. tuber- 

 culosis. 



Variants : 



(a) The KH2PO4 may be omitted. 



(b) Na2C03 naay be substituted for NaCl. 



(c) 2.0 g. of potassium acetate and 2.0 g. 

 of calcium acetate may be added. 



(d) 2.0 g. of ammonium acetate with 

 2.0 g. or 1.0 g. of KH2PO4 may be 

 used. 



Reference: Wherry (1913 pp. 147-148). 



299. Harvey's Ammonium Acetate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Ammonium acetate 10.0 g. 



3. K0HPO4 5.0 g. 



4. MgS04 5.0 g. 



5. Ca3(P04)2 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 

 Reference: Harvey (1921-22 p. 103). 



300. Proskauer and Beck's Basal Ammo- 



nium Tartrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 (0.5%) 5.0 g. 



3. MgS04 (0.25%) 2.5 g. 



4. Ammonium tartrate (0.2%). 2.0 g. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the carbon sources listed. 

 Sterilization: Not specified. 



Use: To study the constituents essential 



for growth of the tubercle bacillus. 

 Added nutrients and variants: 



(a) The authors employed the following 

 carbon sources: 



mannitol 0.6% 



dulcitol 0.6% 



isodulcitol 0.6% 



sucrose 1.0% 



lactose 1.0% 



maltose 1.0%, 



raflfinose 1-0% 



mannose 1.0% 



levulose 1.0% 



(b) Besson used a solution containing 

 10.0 g. ammonium phosphate, 1.0 g. 

 potassium phosphate, 0.2 g. MgS04 

 and 0.12 g. CaCU per liter and added 

 2.0% sucrose or glucose. 

 References: Proskauer and Beck (1894 

 pp. 144-147), Besson (1920 p. 37). 



301. Carapelle's Methylene Blue Ammo- 

 nium Tartrate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Ammonium tartrate 10.0 g. 



3. Potassium phosphate 10.0 g. 



4. MgS04 5.0 g. 



5. Tri-basic calcium phos- 

 phate 5.0 g. 



6. Methylene blue (1.0% 



soln.) several drops 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in (1). 



(2) Tube after sterilization. 



(3) Add several drops of a 1.0% methylene 

 blue solution to each tube. 



Sterilization: Method not given. 



Use: To determine reduction ability of 



bacteria. 

 Variants: One of the following indicators 



may replace methylene blue: 



indigo carmine 



lackmoid 



litmus 



sodium salt of indigo sulphuric acid 

 Reference: Carapelle (1908 p. 552). 



302. Meyers' Basal Ammonium Tartrate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium tartrate 10.0 g. 



3. Anhydrous sodium sulphate. 10.0 g. 

 Preparation : 



(1) Dissolve 2 and 3 in 1. Flask. 



(2) Adjustment of reaction not given. 



(3) After sterilization suspend a sterile 

 strip of filter paper soaked in 10.0% 

 lead acetate solution in each flask. 



Sterilization: Autoclave at 15 pounds pres- 

 sure for 10 minutes. 

 Use: To determine H2S production by 



