CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



87 



colon-typhoid group. No growth of cul- 

 ture studied under aerobic condition. 



Modification: The author added 10.0 g. 

 glucose. 



Reference: iMeyers (1920 p. 248). 



303. Naegeli's Ammonium Tartrate Solution 



(Smith) 

 Constituents: 



1. Water 1000.0 cc. 



2. Ammonium tartrate 10.0 g. 



3. K2HPO4 1.0 g. 



4. MgS04 0.2 g. 



5. CaClj 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Variants : 



(a) Harvey used 0.12 g. CaCU instead 

 of 0.1 g. 



(b) Harvey used 10.0 g. K2HPO4 instead 

 of 1.0 g., 10.0 g. MgS04 instead of 

 0.2 g., and 0.5 g. Ca3(P04)2 instead 

 of 0.1 g. CaCla. 



(c) Harvey used 5.0 g. KH2PO4, 0.5 g. 

 calcium phosphate instead of CaClz 

 and 5.0 g. MgSOi instead of 0.2 g. 



(d) Tanner, Devereux and Higgins used 

 a similar medium with 0.12 g. CaCl2 

 for the cultivation of yeast and yeast- 

 like fungi. 



References: Smith (1905 p. 197), Harvey 

 (1921-22 p. 103), Tanner, Devereu.x and 

 Higgins (1926 p. 62). 



304. Cohn's Ammonium Tartrate Solution 



(Smith) 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. KH2PO4 5.0 g. 



3. MgS04 5.0 g. 



4. Ammonium tartrate 10.0 g. 



5. KCl 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: General culture medium. 

 Variants : 



(a) Roux and Rochaix specified the use 

 of 10.0 g. potassium phosphate in- 

 stead of 5.0 g. KH2PO4 and used 0.5 g. 

 Ca3(P04)2 instead of KCl. 



(b) Kolle and Wassermann did not specify 

 the type of potassium phosphate 

 used, andsubstituted0.5g. Ca3(P04)2 

 for KCl. 



(c) Klimmer omitted the KCl. 



References: Smith (1905 p. 197), Roux and 

 Rochaix (1911 p. 103), Kolle and Wasser- 

 mann (1912 p. 7), Lohnis (1913 p. 44), 

 Tanner (1919 p. 63), Besson (1920 p. 37), 

 Dopter and Sacqu6pee (1921 p. 121), 

 Giltner (192i p. 369), Klimmer (1923 

 p. 172). 



305. Nicolle and Zia Bey's Ammonium 



Lactate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium lactate . . . 10.0 g. 



3. MgS04 2.5 g. 



4. Potassium phosphate . 0.0 or 5.0 g. 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) 5.0 g. of potassium phosphate may 

 or may not be added. 



Sterilization: Not specified. 



Use: To study pigment and fluorescence 

 production by Bacillus pyocyaneiis. 

 Phosphate hastened and increased pig- 

 ment and fluorescence production. 



Reference: Nicolle and Zia Bey (1896 

 p. 671). 



306. Braun and Cahn-Bronner's Ammonium 



Lactate Solution 



Constituents: 



1. Water 1000.0 g. 



2. NaCl 5.0 g. 



3. KH2PO4 2.0 g. 



4. Ammonium lactate 6.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in about 75.0 cc. of 

 water. 



(2) Neutralize (1) with sodium carbonate. 



(3) Dissolve 4 in 25.0 cc. water. 



(4) Boil (2) and (3) for 1 hour to sterilize. 



(5) When cool mix the two solutions. 



(6) Neutralize to litmus by the addition 

 of sterile N/1 soda solution and then 

 add 0.7 cc. sterile N/1 soda solution. 



Sterilization: See step (4) under prepara- 

 tion. 



Use: To study food requirements for colon 

 typhoid group. Authors reported that 

 colon bacillus and para typhoid B become 

 strict aerobes in this medium. 



Variants: Branham used 0.2% K2HPO4 

 instead of KH2PO4 and adjusted the 

 reaction so that the final pH be between 

 6.8 and 7.2. He used the medium for 



