88 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



toxin production by Bacterium enteri- 

 tidis. 

 References: Braun and Cahn-Bronner (1921 

 pp. 4, 197), Branham (1925 p. 299). 



307. Lohnis' Ferric Ammonium Citrate 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Ferric ammonium citrate 

 (0.05%) 0.5 g. 



Preparation : 



(1) Dissolve 0.05% ferric ammonium 

 citrate in water. 



(2) Distribute in test tubes. Fill the 

 tubes. 



Sterilization: Not specified. 



Use: Cultivation of iron bacteria. 



Variants: Brussoff cultivated Ferribac- 

 terium duplex on a medium prepared as 

 follows. He reported that growth ap- 

 peared after several weeks as a very thin 

 yellow membrane. 



(1) Boil tap water. 



(2) Filter. 



(3) Dissolve 0.05% ferric ammonium 

 citrate in (2). 



(4) Distribute in 50.0 cc. lots in small 

 Erlenmeyer flasks. 



(5) Sterilize in the autoclave for 15 to 20 

 minutes under 1.5 atmospheres pres- 

 sure. 



References: Lohnis (1913 p. 116), Brussoff 

 (1916 p. 551). 



308. Gassard's Ammonium Succinate 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Ammonium succinate 10.0 g. 



3. Potassium phosphate 5.0 g. 



4. xMgS04 2.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: To study pigment and fluorescence 

 production by Bacillus pyocyaneus. 

 Also used for tlie cultivation of tubercle 

 bacilli. 

 Variants : 



(a) Tlie author used 1.0 g. ammonium 

 succinate, specified the use of 5.0 g. 

 of Na2HP04 or K2HPO4 and added 

 1.25 g. CaCl2 to the solution. 



(b) Gessard and Vaudremer specified the 



useof K2HPO4, used 5.0 g. ammonium 



succinate and added 1.25 g. CaClj 



for the cultivation of tubercle bacilli. 



References: Gessard (1892 p. 809), (1901 



p. 818); Gessard and Vaudremer (1922 



p. 1012). 



309. Kuntze's Ammonium Succinate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KH2PO4 10.0 g. 



3. MgCls 1.0 g. 



4. Ammonium succinate 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: To study pigment production. 

 Author reported that when traces of 

 H2SO4 were added to the medium pig- 

 ment was produced, otherwise no pig- 

 ment was formed. 



Variants: The author used 1.0 g. K2HPO4 

 instead of 10.0 g. KH2PO4, and from 1.0 

 to 2.0 g. MgS04 instead of 1.0 g. MgCla. 

 The solution was adjusted to neutrality. 

 Bacillus prodigiosus did not produce pig- 

 ment on this medium. 



Reference: Kuntze (1907 p. 306). 



310. Fermi's Basal Ammonium Succinate 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Ammonium succinate 



(0.5 to 1.0%) 5.0 to 10.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. MgS04 (0.5%) 5.0 g. 



5. K3PO4 (0.05%) 0.5 g. 



Preparation : 



(1) Dissolve2, 3, 4and5in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Not specified. 



Use: Cultivation of Schizomycetes . 

 Added nutrients: The author added 5.0% 



glycerol or 5.0% sucrose. 

 Reference: Fermi (1892 p. 26). 



311. Fischer's Glucose Ammonium Tartrate 



Solution (Miiller) 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 (0.1%) 1.0 g. 



3. MgSO4(0.02%) 0.2 g. 



