CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



89 



4. CaClj (0.01%) 0.1 g. 



6. Dextrose (1.0%) 10.0 g. 



6. Ammonium tartrate (amount not 

 given). 

 Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Bad. Giintheri, 

 Reference: Muller (1907 p. 472). 



312. NMgeli's Sucrose Ammonium Tartrate 

 Solution (Fermi) 



Constituents: 



1. Water 1000.0 cc. 



2. Ammonium tartrate 10 g. 



3. KH2PO4 1.0 g. 



4. MgS04 0.2 g. 



5. CaCl2 2.1 g. 



6. Sucrose 50.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Schizomycetes. 

 Reference: Fermi (1892 p. 26). 



313. Groenewege's Sucrose Ammonium 

 Tartrate Solution 



Constituents : 



1. Water 1000.0 ec. 



2. Saccharose 20.0 g. 



3. Sodium ammonium tartrate. 1.0 g. 



4. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Invertase production by Phytobacter 

 lycopersicum n. sp. (causing tomato rot). 

 Reducing sugars are found to be present 

 when tested with Fehling's solution. 



Reference: Groenewege (1913 p. 28). 



314. Fermi and Montesano's Sucrose 

 Ammonium Tartrate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium tartrate 0.5 g 



3. Potassium phosphate 0.5 g 



4. MgS04 0.5 g 



5. Calcium phosphate 0.05 g 



6. Sucrose 5.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute into flasks. 

 Sterilization : Not specified. 



Use: To study inversion of sugar in syn- 

 thetic medium by bacteria, yeasts and 

 molds. 



Variants: Authors substituted 5.0 g. glyc- 

 erol for sucrose. 



Reference: Fermi and Montesano (1895 

 p. 547). 



315. Proskauer and Beck's Glycerol Ammo- 



nium Lactate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol (1.5%) 15.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. MgS04 (0.25%) 2.5 g. 



5. Ammonium lactate (0.5%).. 5.0 g. 

 Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: To study the constituents essential 



for growth of the tubercle bacillus. 

 Variants : 



(a) The authors added 1.0% glucose, 

 0.25% K2SO4 and 0.12% NaCl. 



(b) The authors added 0.3% (NH4)2S04 

 and omitted 0.25% MgSOi. 



(c) Gessard and Vaudremer used 5.0% 

 glycerol and added 0.125% CaClz. 



References: Proskauer and Beck (1894 

 pp. 142, 148), Gessard and Vaudremer 

 (1922 p. 1012). 



316. Stutzer and Hartleb's Glycerol Ammo- 



nium Citrate Solution 

 Constituents: 



1. Water 1000.0 cc 



2. Ammonium citrate 1-0 g. 



3. Glycerol 10.0 g. 



4. Potassium phosphate 10 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of a bacterium from a 

 case of foot and mouth disease. The 

 authors report that the medium was 

 strongly clouded after about 3 weeks. 



Reference: Stutzer and Hartleb (1897 

 p. 382). 



317. Kuntze's Glycerol Ammonium Suc- 



cinate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. MgS04 2.0 g. 



3. KH2PO4 1.0 g. 



