90 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



4. Glycerol (double distilled 

 Merck) 20.0 g. 



5. Ammonium succinate 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjust the reaction to neutral to 

 litmus. 



Sterilization: Method not given. 



Use: To study pigment production by 



Aspergillus niger and Bacillus prodi- 



giosvs. 

 Reference: Kuntze (1907 p. 303). 



318. Wherry's Glycerol Ammonium Acetate 



Solution 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. NaCl 1.0 g. 



3. KCl 1.0 g. 



4. CaCls 1.0 g. 



5. MgS04 1.0 g. 



6. Ammonium acetate 5.0 g. 



7. KH2PO4 2.0 g. 



8. Glycerin 50.0 g. 



(Salts to be Kahlbaum's c.p.) 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Adjustment of reaction not given. 

 Sterilization: Sterilize in autoclave, time 



not given. 



Use: To study acid proof ness of B. tuber- 

 culosis. 



Variants: The author substituted NaCOj 

 for NaCl. 



Reference: Wherry (1913 p. 147). 



319. Proskauer and Beck's Basal Glycerol 



Ammonium Tartrate Solution 



Constituents: 



1. Water 



2. Glycerol (1.5%). 



3. KH2PO4 (0.5%). 



4. MgS04 (0.25%) . 



1000.0 cc. 

 15.0 g. 

 5.0 g. 

 2.5 g. 

 4.0 g. 



5. Ammonium tartrate (0.4%). 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add additional carbon compounds as 

 desired. 



Sterilization: Not specified. 



Use: To study the constituents essential 

 for the growth of the tubercle bacillus. 



Added nutrients and modifications: The 

 authors suggested the following modifi- 

 cations: 



Use of 0.2% ammonium tartrate with the 

 addition of one of the following: 



mannitol 0.6% 



glucose 1.0% 



mannose 1.0% 



raffinose 1.0% 



maltose 1 .0% 



lactose 1.0% 



sucrose 1.0% 



dulcitol 1.0% 



isodulcitol 1.0% 



magnesium citrate 1.0% 



(NH3)2C03 0.3% 



levulose 1.0% 



K2SO4 0.25% 



NaCl 0.12% 



glucose 1 .0% 



Reference: Proskauer and Beck (1894 

 pp. 144-148). 



320. Uschinsky's Glycerol Ammonium Lac- 

 tate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 40.0 to 50.0 g. 



3. NaCl 5.0 to 7.0 g. 



4. CaClj 0.1 g. 



5. MgSOi 0.2 g. 



6. K2HPO4 1.0 g. 



7. Ammonium lactate . . 10.0 g. 

 Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 



7 in 1. 

 Sterilization: Not specified. 

 Use: General synthetic culture medium. 

 Variants: Gratia suggested the following 



solution adjusted to pH = 7.4: 



1. Water 1000.0 g. 



2. Glycerol 30.0 cc. 



3. NaCl 5.0 g. 



4. CaClj 0.1 g. 



5. MgS04 0.2 g. 



6. K2HPO4 2.0 g. 



7. Ammonium lactate 12.0 g. 



Reference: Uschinsky (1893 p. 316), Gratia 



(1922 p. 295), Brieger and Cohn (1893 

 p. 9). 



321. Fermi and Montesano's Glycerol 

 Ammonium Tartrate Solution 



Constituents : 



1. Distilled water 100.0 cc. 



2. Ammonium tartrate 0.5 g. 



3. Potassium phosphate 0.5 g. 



4. MgSOi 0.5 g. 



