CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



91 



5. Calcium phosphate 0.05 g. 



6. Glycerol 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute into flasks. 

 Sterilization: Not specified. 



Use: Cultivation of bacteria, yeast and 



molds. 

 Reference: Fermi and Montesano (1895 



p. 547). 



322. 



Gottheil's Ammonium Tartrate 

 Solution No. VI 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 10 g. 



3. CaClj 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



6. Ammonium tartrate 10.0 g. 



7. Glycerol 10.0 g. 



8. Sucrose 5.0 g. 



9. Iron trace 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 



8 and 9 in 1. 

 Sterilization: Not specified. 

 Use: General culture medium. Author 



used organisms from the soil, and bacteria 



found on roots and rhizomes. 

 Reference: Gottheil (1901 p. 432). 



323. MacKensie's Ammonium Tartrate 

 Solution (Smith) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium tartrate acid. . . 1.5 g. 



3. KH2PO4 2.5 g. 



4. K2SO4 1.5 g. 



5. NaCl 0.5 g. 



6. Glucose 5.0 g. 



7. Lactose 5.0 g. 



8. Glycerol 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Adjust alkaline to phenolphthalein. 

 Sterilization: Not specified. 



Use: General culture medium. 



Variants: Tanner used 2.5 g. acid ammo- 

 nium phosphate and 1.5 g. KH2PO4 

 instead of amounts given by Smith. 



Reference: Smith (1905 p. 198), Tanner 

 (1919 p. 68). 



SUBGROUP I-C. SECTION 3 



Liquid media with constituents of known 

 chemical composition. Nitrogen supplied 

 as nitrites; carbon organic. 

 A I. Organic carbon supplied only as carbo- 

 hydrates. 

 A2. Organic carbon supplied as alcohols. 

 Stutzer and Hartleb's Glycerol 



Nitrite Solution 324 



Beijerinck and van Delden's Alan- 



nitol Nitrite Solution 325 



A3. Organic Carbon supplied only as 

 organic acids. 

 Beijerinck and van Delden's Acetate 



Nitrite Solution 326 



Beijerinck and van Delden's ]\Ialate 

 Nitrite Solution 327 



324. Stutzer and Hartleb's Glycerol Nitrite 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Potassium phosphate 10 g. 



3. NaCl 1.0 g. 



4. MgS04 0.5 g. 



5. KCl 0.1 g. 



6. Glycerol 



7. NaNOa 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 200.0 cc. lots. 



(3) Prepare a 2.0% solution of sodium 

 nitrite. 



(4) After sterilization of (2) and (3) add 

 5.0 cc. of (3) under 4 drops of glycerin 

 to each 200.0 cc. lot of (3) under 

 aseptic conditions. 



(5) Stopper with a double hole rubber 

 stopper so air may be bubbled thru 

 the medium. 



Sterilization: Not specified. 



Use: To study oxidation of nitrites. The 

 author found that after 14 days neither 

 nitrite nor nitrate test could be obtained. 

 Nitrogen was in organic form. If more 

 NaNOo be added after 3 days nitrate was 

 present. Air freed of CO2 was bubbled 

 thru the medium. 



Reference: Stutzer and Hartleb (1897 

 p. 311). 



