92 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



325. Beijerinck and van Delden's Mannitol 



Nitrite Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO1 0.5 g. 



3. NaNOa 0.5 g. 



4. Mannitol 20.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: To study denitrification by B. sub- 

 tilis, B. mesentericus and Azotobacter 

 chroococcum. The authors reported that 

 B. subtilis did not produce ammonia 

 while the other organisms gave ammonia. 



Reference: Beijerinck and van Delden 

 (1902 p. 41). 



326. Beijerinck and van Delden's Acetate 



Nitrite Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. KCl 0.2 g. 



3. K2HPO4 0.2 g. 



4. KNO2 10 g. 



5. Sodium acetate 0.2 g. 



6. Beijerinck and van Del- 

 den's basic salt solution. . . 10 drops 



(See Med. 25) 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 1 drop of Beijerinck and van 

 Delden's Basic Salt Solution (see 

 Med. 25) to each 100.0 cc. of 1. 



(3) Reaction to be slightly alkaline. 

 Sterilization: Not specified. 



Use: Cultivation of Bacillus oligocarbo- 

 philus. The organism grew as a dry thin 

 snow white scum. 



Reference: Beijerinck and van Delden 

 (1903 p. 41). 



327. Beijerinck and van Delden's Malate 



Nitrite Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. NaNOj 0.5 g. 



4. Calcium malate 20.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: To study denitrification by B. sub- 

 tilis, B. mesentericus and Azotobacter 

 chroococcum. The authors reported that 

 NH3 was formed only by Azotobacter 

 chroococcum. 



Reference: Beijerinck and van Delden 

 (1902 p. 41). 



SUBGROUP 1-C. SECTION 4 



Liquid media or basal solutions of known 



chemical composition. Nitrogen supplied 



as nitrates; carbon organic. 



Ai. Incomplete or basal solutions, em- 

 ployed with the addition of other nu- 

 trients. 



Stutzer's Basal Citrate Nitrate Solu- 

 tion 328 



Mortensen's Basal Nitrate Salt Solu- 

 tion 329 



A2. Complete media. 



Bi.* Only one type of organic carbon 

 supplied. 



Ci.* Organic carbon supplied as carbo- 

 hydrates. 



Di. Monosaccharides, only, added. 



Ei. Containing inorganic salts of mono- 

 valent cations only. 



Stutzer's Glucose Nitrate Solution. . 330 

 Buhlert and Fickendey's Glucose 

 Nitrate Solution 331 



E2. Containing inorganic salts of mono and 

 divalent cations. 



Fi. Primarily for the cultivation of bac- 

 teria. 

 Beijerinck and Minkman's Glucose 



Nitrate Solution 332 



Maze's Glucose Nitrate Solution. . . . 333 

 Fischer's Glucose Nitrate Solution 



(Miiller) 334 



Committee S. A. B. Glucose Nitrate 



Solution 335 



Heraeus' Glucose Nitrate Solution.. 336 

 Migula's Glucose Nitrate Solution.. 337 

 Wherry's Levulose Nitrate Solution. 338 



F2. Not primarily for cultivation of bac- 

 teria. 



Went's Glucose Nitrate Solution 339 



Charpentier's Glucose Nitrate Solu- 

 tion 340 



E.2 Inorganic salts of mono, di and tri- 

 valent cations added. 

 Doryland's Glucose Nitrate Solution . 341 



D2. Disaccharides, only, added. 

 Bokorny's Sucrose Nitrate Solution. 342 

 Stoklasa and Vitek's Sucrose Ni- 

 trate Solution 343 



Czapek's Sucrose Nitrate Solution.. 344 



* See page 93 for B2 and C2. 



