CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



93 



D3. Polysaccharides, only, added. 

 Gerlach and Vogel's Cellulose Nitrate 



Solution 345 



Dubos' Cellulose Sodium Nitrate 



Solution 346 



Krainsky's Starch Nitrate Solution. 347 

 Lohnis' Cellulose Nitrate Solution. . . 348 

 C2. Organic carbon supplied only as alco- 

 hols. 

 Di. Monoatomic alcohols, only, added. 

 Beijerinck and Minkman's Alcohol 



Nitrate Solution 349 



Bokorny's Alcohol Nitrate Solution. 350 

 D2. Polyatomic alcohols, only, added. 

 Kuhne's Glycerol Nitrate Solution 



(Proskauer and Beck) 351 



Weissenberg's Glycerol Nitrate Solu- 

 tion 352 



Lantzsch's Glycerol Nitrate Solution. 353 

 Stoklasa's Mannitol Nitrate Solution. 354 

 C3. Organic C;arbon supplied as acids or 

 their salts. 



Doryland's Acetic Acid Nitrate Solu- 

 tion 355 



Giltay and Aberson's Citrate Nitrate 



Solution (Murray) 356 



Percival's Tartrate Nitrate Solution. 357 

 B2. More than one type of organic carbon 



supplied. 

 Ci. Carbon added as carbohydrates and 

 alcohols. 



Gottheil's Nitrate Solution No. VII. 358 

 C2. Carbon added as carbohydrates and 

 acids. 

 Burri and Stutzer's Nitrate Solution. 359 



Kuntze's Nitrate Solution 360 



Giltay and Aberson's Nitrate Solu- 

 tion (Stoklasa and Vitek) 361 



Giltay's Sucrose Nitrate Solution 



(Fred) 362 



Behrens' Cellulose Nitrate Solution. 363 

 C3. Carbon added as alcohols and organic 

 • acids. 

 Maassen's Malic Acid Nitrate Solu- 

 tion (Stoklasa and Vitek) 364 



Maassen's Stearate^Nitrate Solution 

 (Vierling) 365 



328. Stutzer's Basal Citrate Nitrate 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Potassium citrate 10.0 g. 



3. KNO3 3.0 g. 



4. K2HPO4 1.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the organic nitrogen com- 

 pounds given in added nutrients. 



(3) Reaction to be slightly alkaline. 



(4) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification by B. agilis, 

 B. nitrovorus, B. Stutzeri, B. Hartlebi. 

 Author found that nitrate reaction dis- 

 appeared after 6 to 8 days. When pep- 

 tone or meat extract was added B. Stut- 

 zeri and B. Hartlebi decomposed all the 

 nitrate in 3 days. B. nitrovorus and 

 B. agilis after 6 to 7 days. 



Added nutrients: Author suggested the 

 addition of peptone or Schiilke and 

 Myers beef extract, or a mixture of the 

 two. Amounts not specified. 



Variants: The author used the basic solu- 

 tion without additions. 



Reference: Stutzer (1901 p. 85). 



329. 



Mortensen's Basal Nitrate Salt 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KNO3 (0.5%) 5.0 g. 



3. Cobalt salt 

 Preparation : 



(1) Dissolve 2 and 10.0% of one of the 

 carbon sources listed under added 

 nutrients in 1. 



(2) Add cobalt salts in varying amounts 

 from 4 to 1/32%. 



Sterilization: Not specified. 



Use: To determine the toxic properties of 

 cobalt salts for Aspergillus niger. The 

 author found the toxic properties of 

 cobalt were different in different media. 

 A 0.5% cobalt chloride solution in a 

 liquid medium was as toxic as 1.0% 

 cobalt chloride in a gelatin medium. 



Added nutrients: The author added 10.0% 

 of one of the following to the basic 

 solution: 

 glucose 

 sucrose 

 glycerol 



Reference: Mortensen (1909 p, 523). 



