94 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



330. Stutzer's Glucose Nitrate Solution 



Constituents: 



1. Water 



2. Glucose 



3. KNO3 

 Preparation : 



(1) Dissolve 2 and 3 in 1. (Amounts 

 not specified.) 



(2) Reaction to be slightly alkaline. 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification by B. agilis, 

 B. nitrovorus, B. Slutzeri and B. Hart- 

 lebi. Nitrate was not reduced. 



Variants: Beijerinck and Minkman used a 

 2.0% glucose with 1.0% KNO3 solution 

 to study denitrification. They found a 

 butyric acid fermentation took place 

 when inoculated with soil. 



Reference: Stutzer (1901 p. 84), Beijerinck 

 and Minkman (1910 p. 35). 



331. Buhlert and Fickendey's Glucose 



Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose (1.5%) 15.0 g. 



3. NaNOa (0.3%) 3.0 g. 



4. Potassium phosphate (0.1%). 1.0 g. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute in 10.0 cc. lots. 



(3) Inoculate each tube with 5.0 cc. soil 

 water. 



Sterilization: Not specified. 

 Use: To study denitrification. 

 Variants: 



(a) Barthel specified the use of 0.1% 

 K3PO4. 



(b) Percival and Harvey specified the use 

 of tap water and usedO.1% K2HPO4. 



References: Buhlert and Fickendey (1906 

 p. 402), Barthel (1910 p. 118), Percival 

 (1920 p. 202), Harvey (1921-22 p. 108). 



332. Beijerinck and Minkman's Glucose 



Nitrate Solution 

 Constituents: 



1. Water 100.0 cc. 



2. Glucose 2.0 g. 



3. Chalk 2.0 g. 



4. KNO3 1.0 g. 



5. NH-Cl 0.05 g. 



6. K2HPO4 0.05 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Inoculate with soil and incubate in a 

 closed flask. 



Sterilization: Not specified. 



Use: To study denitrification. The au- 

 thors found that 53.0% CO 2, 32.0% H2 

 and 15.0% N2 were formed if nitrate was 

 present. Without nitrate 50.0% of the 

 gas formed was CO2 and 50.0% H2. 



Reference: Beijerinck and Minkman (1910 

 p. 35). 



333. Maze's Glucose Nitrate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1.0 g. 



3. MgS04 0.5 g. 



4. FeS04 0.02 g. 



5. NaCl 0.02 g. 



6. CaC03 1.0 g. 



7. Glucose 20.0 g. 



8. NaNOa 5.0 g. 



Preparation : (1) Dissolve 2, 3, 4, 5, 6, 7 and 



8 in 1. 



Sterilization: Not specified. 



Use: To study denitrification by Bacillus 

 denitrificans . The author reported the 

 formation of nitrous acid in 24 hours. 



Variants : Percival used the following solu- 

 tion to study denitrification by B. 

 mycoides: 



1. Water 1000.0 g. 



2. K2HPO4 50 g. 



3. MgS04 2.5 g. 



4. NaCl 2.5 g. 



5. Glucose 2.0 g. 



6. NaNOs 2.0 g. 



References: Maze (1911 p. 370), Percival 



(1921 p. 112). 



334. Fischer's Glucose Nitrate Solution 



(Miiller) 



Constituents: 



1. Water 



2. K0HPO4 (0.1%) 



3. MgS04 (0.02%) 



4. CaCl2 (0.01%) 



5. Dextrose (1.0%) 



1000.0 cc. 



1.0 g. 



0.2 g. 



0.1 g. 



10.0 g. 



6. KNO3 (amount not specified). 

 Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To study denitrification by Bacleriuin 



