CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



95 



Giintheri, Bad. Hartlebi, Bac. pyo- 

 cyaneous and Bac. fluorescens. 

 Variants: von Caron used the following 

 solution to study denitrification: 



1. Water 1000.0 cc. 



2. Glucose 1.0 g. 



3. KNO3 0.1 or 3.0 g. 



4. MgS04 2.0 g. 



5. K2HPO4 2.0 g. 



6. CaCla 0.2 g. 



References : MuUer (1907 p. 472), von Caron 



(1912 p. 96). 



335. Committee S. A. B. Glucose Nitrate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. CaCIs 0.5 g. 



3. Nitrate (kind not given). . . 1.0 g. 



4. Glucose 10.0 g. 



5. K2HPO4 0.5 g. 



Preparation : (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Method not given. 



Use: To study reduction of nitrates. Test 

 for nitrite with sulphanilic acid and a- 

 naphthylamine and for ammonia produc- 

 tion by Thomas' method. 



Variants: Committee S. A. B. specified the 

 useof l.Og. KNO3. 



Reference: Committee S. A. B. (1922 

 p. 525), (1923 p. 27). 



336. Heraeus' Glucose Nitrate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 0.1 g. 



3. MgS04 0.2 g. 



4. CaCls 0.01 g. 



5. Glucose 0.8 g. 



6. Ca(N03)2 0.8 g. 



7. (NH4)2C03 

 Preparation : 



(1) Dissolve 1.0 g. potassium phosphate, 

 0.2 g. MgS04 and 0.1 g. CaCl. in 

 1000.0 g. distilled water. 



(2) Steam for 1 hour. 



(3) Allow to stand. 



(4) Filter and boil again. 



(5) Prepare a solution of Ca(N03)2 by 

 treating a weighed amount of CaC03 

 with dilute HNO3 the solutions being 

 warm. Be sure that there is no free 

 HNO3 in solution. There should be 

 some undissolved salt present. Then 



add some more CaCOs. This removes 

 all traces of HXO3. 



(6) Steam in a weighted porcelain dish, 

 until a solution is obtained contain- 

 ing 1 part Ca(N03)2 to five parts 

 water. 



(7) Filter and store. 



(8) Prepare solutions of dextrose and 

 (NH4)2C03 the ratio of 1:15. 



(9) Mix 25.0 cc. (4), 1.0 cc. of the dextrose 

 solution, 2 drops of the (NH4)2C03 

 solution and 2 drops of (7). 



(10) Dilute the 250.0 cc. with distilled 

 water. A liter of this solution con- 

 tains: 



potassium phosphate 0.1 g 



MgS04 0.02 g 



CaCl2 0.01 g 



dextrose 0.8 g 



Ca(N03)2 0.8 g 



(11) Distribute in 50.0 cc. flasks that have 

 previously been plugged and steri- 

 lized. 



Sterilization: Heat the solution and the 

 flasks in the steamer for 30 minutes. 



Use: Culture medium for soil, water and 

 air forms. 



Reference: Heraeus (1896 p. 216). 



337. Migula's Glucose Nitrate Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. KNO3 10.0 g. 



3. Magnesium phosphate 2.0 g. 



4. CaCl2 l.Og. 



5. K2HPO4 l.Og. 



6. Glucose 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Tube. 



Sterilization: Boil in the steamer on two 



successive days. 

 Use: Differentiation of typhoid bacilli 



from B. coll. 

 Reference: Migula (1901 p. 20). 



338. Wherry's Levulose Nitrate Solution 



Constituents: 



1. Redistilled water 



2. Na2C03 



3. KCl 



4. CaCl2 



5. MgS04 



6. KNO3 



1000.0 cc. 

 l.Og. 

 l.Og. 

 l.Og. 

 l.Og. 

 4.0 g. 



