96 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



7. KjHPO* 2.0 g. 



8. Levulose 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Adjustment of reaction not given. 

 Sterilization: Sterilize in autoclave, time 



not given. 



Use: To study acid proofness of B. tubercu- 

 losis. Luxuriant growth with K2HPO4, 

 but no growth without K2HPO4. Acid 

 fast properties of organism not given. 



Reference: Wherry (1913 p. 151). 



339. Went's Glucose Nitrate Solution 



Constituents : 



1. Water 1000.0 g. 



2. Glucose (Commercial) 50.0 g. 



3. KNO3 5.0 g. 



4. KH2PO4 1.0 g. 



5. AIgS04 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjustment of reaction not specified. 

 Sterilization: Method not given. 



Use: Cultivation of Monilia sitophila 

 (Mont) Sacc. Organism seems to grow 

 best in damp atmosphere. Also grows 

 well on rice, arachis seeds, bread, roots 

 of Daucus carota, milk, etc. 



Reference: Went (1901 p. 546). 



340. Charpentier's Glucose Nitrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04 1.0 g. 



3. K2HPO4 2.0 g. 



4. KNO3 2.0 g. 



5. CaNOa 0.05 g. 



6. FeS04 trace 



7. Glucose 10.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Heat at 120°C. 



(3) Filter. 



Sterilization: Sterilize at 120°C. 



Use: To determine nitrate reduction by 

 Algae, Cystococcus humicola. The 

 author reported that the total nitrogen 

 content is raised after 13 days incubation. 



Variant: The author omitted the 2.0 g. 

 KNO3 and added 1.0 g. of Ca(N03)2 in- 

 stead of only 0.05 g. 



Reference: Charpentier (1903 p. 327). 



341. Doryland's Glucose Nitrate Solution 

 Constituents: 



1. Distilled water 500.0 cc. 



2. HCl Dilute 



3. MgS04 0.5 g. 



4. CaO 0.01 g. 



5. Fe2(S04)3 0.01 g. 



6. MnS04 0.01 g. 



7. HNO3 (amount not given) 



8. H2SO4 



9. H3PO4 



10. Glucose 5.0 g. 



11. N/0.2578 NaOH 



12. N/0.6205 KOH 

 Preparation : 



(1) Dilute HCl so that 1.0 cc. is not quite 

 neutralized by 1.0 cc. of silicate solu- 

 tion made by dissolving 24.0 g. K2Si03 

 and 8.4 g. NajSiOs in 500.0 g. distilled 

 water. Phenolphthalein as indicator. 



(2) Add to HCl the following salts. 



MgSOi. 0.5 g. 



CaO 0.01 g. 



Fe2(S04)3 0.01 g. 



MnS04 0.01 g. 



HNO3 (amount not specified). 



(3) Standardize (2) against silicate solu- 

 tion so that 1.0 cc. is equivalent to 

 1.0 cc. using methyl orange as 

 indicator. 



(4) Standardize a solution of H2SO4 in 

 same way as HCl omitting the salts. 



(5) Standardize H3PO4 in similar manner 

 as HCl omitting the salts and using 

 phenolphthalein as indicator. 



(6) Mix the acids in the following ratio: 



HCl 153.5 cc. 



H2SO4 77.0 cc. 



H,P04 116.0 cc. 



(7) Mix equal quantities of N/0.6205 

 KOH and N/0.2578 NaOH. 



(8) 1.0 cc. of (7) should neutralize 1.0 

 cc. of (6) using phenolphthalein as 

 indicator. 



(9) Draw acid and base solution in 

 separate burettes and allow to stand 

 several hours to sterilize. 



(10) Add enough sterile glucose solution 

 to a mixture of equal parts of (6) 

 and (7) to give 10 g. of glucose per 

 liter solution. 



Sterilization: Method not given. 



Use: A general synthetic medium. 



Reference: Doryland (1916 pp. 146-148). 



