CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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342. Bokorny's Sucrose Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KNO3 (0.4%) 4.0 g. 



3. Sucrose (10.0%) 100.0 g. 



4. Phosphoric acid (PaOs) 

 (0.13%) 1.3 g. 



Preparation: (1) Dissolve 2, 3, 4 in 1. 



Sterilization: Not specified. 



Use: Cultivation of molds. 



Variants: Bottger used 0.1% K2HPO4 in- 

 stead of phosphoric acid and used 0.2, 

 0.5 or 1.0% KNO3 to determine the 

 toxicity of nitrates for yeast. He re- 

 ported that nitrate presence had little 

 effect on loss of weight, measured in 

 grams. 



Reference: Bokorny (1917 p. 316), Bottger 

 (1921 p. 224). 



343. Stoklasa and Vitek's Sucrose Nitrate 

 Solution 



Constituents: 



1. Water 



2. K2HPO4 1.25 g. 



3. K2SO4 0.2 g. 



4. CaClj 0.05 g. 



5. MgCl2 0.05 g. 



6. NajCOa 0.1 g. 



7. reP04 0.05N 



8. Saccharose 5.0 g. 



9. NaNOs 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1 liter 

 of water. 



(2) Add 8 and 9 to 50.0 cc. of (1). 



(3) Make (2) up to 500.0 cc. 

 Sterilization: Not specified. 



Use: To study nitrate reduction by Clos- 

 tridium gelalinosum, Bad. Hartlebi. 

 The authors reported that when more 

 saccharose was added more nitrate was 

 left unchanged. 



Variants : 



(a) The authors suggested the addition 

 of 2.0 g. of CaCOs with 5.0 or 17.1 g. 

 sucrose and 2.0 or 4.25 g. NaNOa per 



50.0 cc. solution. 



(b) The authors suggested that 5.0 or 



17.1 g. sucrose be added with 2.0 or 

 4.25 g. NaNOs per 50.0 cc. solution. 



(c) The authors added 2.0 g. sucrose and 



1.0 or 2.0 g. of NH4NO3 to 100.0 cc. 

 of solution. 

 Reference: Stoklasa and Vitek (1905 

 p. 198). 



344. Czapek's Sucrose Nitrate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaNOs 2.0 g. 



3. K2HPO4 1.0 g. 



4. KCl 0.5 g. 



5. MgS04 0.5 g. 



6. FeS04 0.01 g. 



7. Sucrose 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Place in 100.0 cc. portions in flasks. 

 Plugg. 



(3) Adjustment of reaction, not given. 

 Sterilization: Sterilize at 15 pounds for 15 



minutes. 



Use: Cultivation of soil forms. 



Variants: Tanner specified the use of 0.5 g. 

 MgS04-7H20. 



References: Waksman (1918 p. 479), Tan- 

 ner (1919 p. 66). 



345. Gerlach and Vogel's Cellulose Nitrate 



Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. NaNOs 5.0 g. 



3. Potassium phosphate 0.5 g. 



4. MgS04 0.3 g. 



5. NaCl 0.5 g. 



6. NaHCOs 0.5 g. 



7. FeS04 some 



8. Straw 25.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in 200.0 cc. lots. 



(3) To each flask add 5.0 g. straw. 



(4) Adjustment of reaction not given. 

 Sterilization: Sterilize in the steamer. 

 Use: Cultivation of albumin formers from 



soil and stable manure. The author 

 found that if the medium was inoculated 

 with albumin formers alone all the 

 nitrate nitrogen was changed to albu- 

 minous nitrogen. When denitrifying bac- 

 teria were inoculated with the albumin 

 formers some of the nitrogen was lost. 

 Reference : Gerlach and Vogel (1901 p. 619). 



