CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



346. Dubos' Cellulose Sodium Nitrate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaNOa 0.5 g. 



3. K2HPO4 1.0 g. 



4. MgS04 0.5 g. 



5. KCl 0.5 g. 



6. FeS04 0.01 g. 



7. Filter paper 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Tube in 5 cc. quantities in tubes con- 

 taining a strip of filter paper. The 

 paper is partially immersed in water. 



Sterilization: Not specified. 

 Use: Isolation of aerobic bacteria decom- 

 posing cellulose. 

 Reference: Dubos (1927 p. 52). 



347. Krainsky's Starch Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Starch (1.0%) 10.0 g. 



3. KNO3 (0.3%) 3.0 g. 



4. K2HPO4 (0.05%) 0.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Enrichment of Actinomycetes coeli- 



color, Actinomycetes diastaticus. 

 Reference: Krainsky (1914 p. 658). 



348. Lohnis' Cellulose Nitrate Solution 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Cellulose 20.0 g. 



3. KNO3 2.5 g. 



4. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 3, 4 and 20.0 g. 



of cellulose (filter paper or cotton wool) 



in 1. 

 Sterilization: Not specified. 

 Use: To study denitrification. 

 Reference: Lohnis (1913 p. HI). 



349. Beijerinck and Minkman's Alcohol 

 Nitrate Solution 



Constituents : 



1. Water 



2. Ethyl alcohol. 



1000.0 cc. 

 5.0 g. 



3. KNO3 10.0 g. 



4. K2HPO4 0.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Inoculate with soil. 



(3) Incubate under anaerobic conditions. 

 Sterilization: Not specified. 



Use: To study denitrification by soil 

 forms, and Bacillus pyocyaneus. The 

 author reported that the medium became 

 blue green due to Bacillus pyocyaneus. 



Variants: Tanner used 5.0 cc. of ethyl 

 alcohol and specified the use of tap water. 



References : Beijerinck and Minkman (1910 

 p. 35), Tanner (1919 p. 67). 



350. Bokomy's Alcohol Nitrate Solution 



Constituents: 



1. Water 



2. Methyl alcohol 



3. KH2PO4 



4. MgS04 



5. Ca(N03)2 

 Preparation : 



(1) Prepare a mineral solution by dissolv- 

 ing 0.05% KH2PO4, 0.025% bitter 

 salts and 0.04% Ca(N03)2 in water. 



(2) Prepare solutions with 0.1% (1) and 

 1.0% methyl alcohol. 



Sterilization: Not specified. 



Use: Cultivation of fungus utilizing methyl 



alcohol as carbon source. 

 Variants: The author used 10.0, 5.0, 2.0, 



0.5, 0.005 or 0.0025% methyl alcohol 



instead of 1.0%. 

 Reference: Bokorny (1911 p. 178). 



351. Kiihne's Glycerol Nitrate Solution 

 (Proskauer and Beck) 

 Constituents: 



1. Water 1000.0 cc. 



2. Ca(N03)2 2.0 g. 



3. KCl 0.5 g. 



4. MgS04 0.5 g. 



5. KH2PO4 0.5 g. 



6. Glycerol 30.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of tubercle bacilli. 



Growth was very scant. 

 Reference: Proskauer and Beck (1894 



p. 131). 



